Suzuki H, Nakayama S, Oshima M, Yoshida Y
Nihon Sanka Fujinka Gakkai Zasshi. 1982 Jun;34(6):761-8.
A scanning electron microscopic study on the human endometrium, using Evan's method and modifications, revealed the following findings. a) 8N . HCl-Collagenase method: High-speed digestion of the specimen made the determination of the adequate digestion time difficult, due to conc. HCl and high processing temperature. b) 1N . HCl-Collagenase method: Long digestion time produced many artifacts as shrinkage of the cells or formation of small holes on them. c) Tripsin-Collagenase method: Tissue breaking often resulted before the adequate digestion. d) Hyaluronidase-Collagenase method: This is most satisfactory in classifying glands and vessels, and digestion time is the shortest of all methods.
一项采用埃文斯方法及其改良方法对人子宫内膜进行的扫描电子显微镜研究，得出了以下结果。a) 8N盐酸 - 胶原酶法：由于浓盐酸和较高的处理温度，对标本进行高速消化使得确定适当的消化时间变得困难。b) 1N盐酸 - 胶原酶法：较长的消化时间产生了许多伪像，如细胞收缩或细胞上形成小孔。c) 胰蛋白酶 - 胶原酶法：在充分消化之前常常导致组织破碎。d) 透明质酸酶 - 胶原酶法：在对腺体和血管进行分类方面最为令人满意，并且在所有方法中消化时间最短。
