Shuttleworth T J
J Exp Zool. 1982 Jun 10;221(2):255-8. doi: 10.1002/jez.1402210218.
In slices of the dogfish rectal gland, amphotericin B produced increases in oxygen consumption and ouabain binding similar to those produced by cyclic AMP, except that the increases induced by the latter were inhibited by furosemide whereas those resulting from the addition of amphotericin B were unaffected by the diuretic. Unlike cyclic AMP, however, amphotericin B failed to stimulate secretion by the isolated perfused gland. These results support the suggestion that the increases in ouabain binding and oxygen consumption produced by cyclic AMP result simply from an increased sodium entry into the cells, and as such can be mimicked by amphotericin B, whereas secretion rate itself depends on some additional, cyclic AMP-induced process. The implications of this for determinations of the relationship between rates of oxygen consumption and ion transport are discussed.
在角鲨直肠腺切片中，两性霉素B使耗氧量和哇巴因结合增加，类似于环磷酸腺苷（cAMP）所产生的增加，只是后者所诱导的增加被呋塞米抑制，而添加两性霉素B所导致的增加不受该利尿剂影响。然而，与环磷酸腺苷不同，两性霉素B未能刺激离体灌注腺的分泌。这些结果支持了以下观点：环磷酸腺苷所产生的哇巴因结合和耗氧量增加仅仅是由于进入细胞的钠增加所致，因此可被两性霉素B模拟，而分泌速率本身则取决于环磷酸腺苷诱导的一些额外过程。本文讨论了这对于确定耗氧率与离子转运之间关系的意义。
