Durnford J M, Scanlon P A, Glaser R
J Gen Virol. 1982 May;60(Pt 1):39-48. doi: 10.1099/0022-1317-60-1-39.
The Burkitt lymphoma/somatic cell hybrid D98/HR-1 contains repressed Epstein-Barr virus (EBV) genomes which can be induced by 5-iodo-2'-deoxyuridine (IdUrd) to express the EBV early antigen (EA) and the virus capsid antigen (VCA). Nuclear and cytoplasmic fractions prepared from uninduced, partially induced and fully induced hybrid cells were radioimmunoprecipitated with human sera having high antibody titres against EA and VCA. Three polypeptides, with mol. wt. 127 x 10(3), 115 x 10(3) and 84 x 10(3) (127K, 115K and 84K), were detected in cells induced to express EA. All three polypeptides continued to be synthesized late in infection. One of the polypeptides was predominantly nuclear, one predominantly cytoplasmic, and one found in both fractions. Three polypeptides, with mol. wt. 144K, 137K and 50K, were detectable only in cells induced to express both EA and VCA. All three polypeptides were found predominantly in the nuclear fraction.
伯基特淋巴瘤/体细胞杂种D98/HR-1含有受抑制的爱泼斯坦-巴尔病毒（EBV）基因组，该基因组可被5-碘-2'-脱氧尿苷（IdUrd）诱导表达EBV早期抗原（EA）和病毒衣壳抗原（VCA）。用针对EA和VCA具有高抗体滴度的人血清对从未诱导、部分诱导和完全诱导的杂种细胞制备的核和细胞质组分进行放射免疫沉淀。在诱导表达EA的细胞中检测到三种分子量分别为127×10³、115×10³和84×10³（127K、115K和84K）的多肽。在感染后期这三种多肽仍持续合成。其中一种多肽主要存在于细胞核中，一种主要存在于细胞质中，还有一种在两个组分中都有发现。三种分子量分别为144K、137K和50K的多肽仅在诱导表达EA和VCA的细胞中可检测到。这三种多肽主要存在于核组分中。
