大鼠脑原代神经细胞培养物中2',3'-环核苷酸3'-磷酸二酯酶（CNPase）的活性及免疫细胞化学定位
Activity and immunocytochemical localization of 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) in primary nerve cell cultures from rat brain.
作者信息
Müller H W, Seifert W
出版信息
Cell Mol Neurobiol. 1982 Sep;2(3):241-7. doi: 10.1007/BF00711151.
Abstract
- In the present investigation we demonstrate the occurrence and immunocytochemical localization of 2′,3′-cyclic nucleotide 3′-phosphodiesterase (CNPase; EC 3.1.4.37) in primary nerve cell cultures from rat brain. 2. Using a specific antiserum to purified CNPase the enzyme has been detected by immunofluorescence staining on the cell surface as well as within the cell body and processes of oligodendrocytes. 3. Substantial amounts of CNPase activity are expressed in these cells prior to the onset of myelin formation. 4. The enzyme activity rapidly increases severalfold in culture during developmental stages corresponding to the period around birth. 5. In contrast to neuronal tumor cell lines [Müller, H. W., (1981). 947–955; Müller, H. W., and Seifert, W. (1982). 227–239], primary neurons from rat brain do not synthesize the enzyme.
摘要
- 在本研究中，我们证明了2′,3′-环核苷酸3′-磷酸二酯酶（CNPase；EC 3.1.4.37）在大鼠脑原代神经细胞培养物中的存在及免疫细胞化学定位。2. 使用针对纯化CNPase的特异性抗血清，通过免疫荧光染色在少突胶质细胞的细胞表面以及细胞体和突起内检测到了该酶。3. 在髓鞘形成开始之前，这些细胞中就表达了大量的CNPase活性。4. 在与出生前后时期相对应的发育阶段，培养物中的酶活性迅速增加了几倍。5. 与神经元肿瘤细胞系不同[米勒，H. W.，（1981年）。947 - 955；米勒，H. W.，和赛弗特，W.（1982年）。227 - 239]，大鼠脑的原代神经元不合成该酶。
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