采用免疫荧光法在微孔板中对爱泼斯坦-巴尔病毒衣壳抗原IgM和EB核抗原IgG抗体进行滴定。一种基于微量滴定系统的半自动方法。
Epstein-barr virus VCA IgM and EBNA IgG antibodies titered by immunofluorescence in microplates. A semi-automated method based on microtiter system.
作者信息
Lamy M E, Favart A M, Leclercq M F, Segas M, Mendez M, Lhoir C, Cornu C, Burtonboy G
出版信息
Med Microbiol Immunol. 1982;170(4):247-53. doi: 10.1007/BF02123315.
We describe methods of immunofluorescence in microplates for titration of EBV VCA-IgM and EBNA-IgG antibodies and compare the sensitivity of the methods with glass slides and with microplates, showing the reproducibility of the methods. Results of VCA-IgM and -IgG, EA and EBNA titers obtained in four groups of patients are given, comprising cases of infectious mononucleosis, renal graft recipients, lymphoproliferative diseases and controls.
我们描述了微孔板中免疫荧光法用于滴定EBV VCA-IgM和EBNA-IgG抗体的方法，并将这些方法与载玻片和微孔板的灵敏度进行比较，展示了这些方法的可重复性。给出了四组患者中获得的VCA-IgM和-IgG、EA和EBNA滴度的结果，这四组患者包括传染性单核细胞增多症、肾移植受者、淋巴增殖性疾病患者及对照组。
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