Fried H M, Warner J R
Nucleic Acids Res. 1982 May 25;10(10):3133-48. doi: 10.1093/nar/10.10.3133.
A cosmid clone bank of yeast DNA has been used to isolate the cycloheximide resistance gene cyh2 of Saccharomyces cerevisiae. A cosmid carrying this gene was identified by cross hybridization to another cloned gene, tsm437. The two genes, which are tightly linked genetically are both present on a 31 kb segment of cloned DNA. The cyh2 gene encodes ribosomal protein L29, a component of the large subunit. Blot hybridization analysis reveals that this gene is present as a single copy in the yeast genome, unlike many other yeast ribosomal protein genes which appear to be duplicated. The cyh2 gene also appears to contain an intervening sequence, a characteristic common to most yeast ribosomal protein genes that have been cloned.
一个酵母DNA黏粒克隆文库已被用于分离酿酒酵母的环己酰亚胺抗性基因cyh2。通过与另一个克隆基因tsm437的交叉杂交，鉴定出携带该基因的黏粒。这两个在遗传上紧密连锁的基因都存在于一段31 kb的克隆DNA片段上。cyh2基因编码核糖体蛋白L29，它是大亚基的一个组成部分。印迹杂交分析表明，与许多其他似乎是重复的酵母核糖体蛋白基因不同，该基因在酵母基因组中以单拷贝形式存在。cyh2基因似乎也含有一个间隔序列，这是大多数已克隆的酵母核糖体蛋白基因共有的特征。
