Dehoux P, Davies J, Cannon M
Unité de Génie Microbiologique, Institut Pasteur, Paris, France.
Eur J Biochem. 1993 Apr 15;213(2):841-8. doi: 10.1111/j.1432-1033.1993.tb17827.x.
The yeast Kluyveromyces lactis is resistant to high concentrations (1 mg/ml) of the antibiotic cycloheximide. Using in vitro translation studies it was confirmed that this extreme resistance is a property of ribosomes. The resistance determinant from K. lactis was cloned into Saccharomyces cerevisiae. Nucleotide sequence analysis of the determinant demonstrated that resistance was conferred by the K. lactis ribosomal protein L41. K. lactis was shown to contain only one copy of the gene that encodes this protein and the gene was located to chromosome III. In contrast, S. cerevisiae was found to contain multiple copies of the gene for the corresponding ribosomal protein L41 which mapped to two of the three chromosomes V, XIV and VIII. Since the cycloheximide-resistance gene of K. lactis causes essentially complete protection against inhibition by the drug, it is likely to be particularly useful as a selective marker in eukaryotic gene transfer studies.
乳酸克鲁维酵母对高浓度（1毫克/毫升）的抗生素放线菌酮具有抗性。通过体外翻译研究证实，这种极端抗性是核糖体的一种特性。将乳酸克鲁维酵母的抗性决定因素克隆到酿酒酵母中。对该决定因素的核苷酸序列分析表明，抗性是由乳酸克鲁维酵母核糖体蛋白L41赋予的。结果表明，乳酸克鲁维酵母仅含有编码该蛋白的基因的一个拷贝，该基因位于第三条染色体上。相比之下，发现酿酒酵母含有对应核糖体蛋白L41的基因的多个拷贝，这些拷贝定位于五条染色体中的两条，即第五、十四和八条染色体。由于乳酸克鲁维酵母的放线菌酮抗性基因能对药物抑制产生基本完全的保护作用，因此它在真核基因转移研究中作为选择标记可能特别有用。
