Jat P, Arrand J R
Nucleic Acids Res. 1982 Jun 11;10(11):3407-25. doi: 10.1093/nar/10.11.3407.
Cloned DNA from the EcoRI J fragment of EBV has been used as template for in vitro transcription experiments using cell-free extracts prepared from HeLa or KB cells. Two EBV specific RNAs each about 175 bases in length were synthesised and nuclease 51 mapping experiments determined that these in vitro products corresponded precisely to the in vivo species obtained from Raji cells. These two RNA molecules are transcribed by RNA polymerase III and in common with other pol III-synthesised RNAs the coding sequences contain intragenic control regions. The relative abundance of the two RNAs synthesised in vitro differs from that observed in vivo.
来自EBV的EcoRI J片段的克隆DNA已被用作体外转录实验的模板，该实验使用从HeLa或KB细胞制备的无细胞提取物。合成了两种长度约为175个碱基的EBV特异性RNA，核酸酶S1作图实验确定这些体外产物与从Raji细胞获得的体内产物精确对应。这两种RNA分子由RNA聚合酶III转录，并且与其他由聚合酶III合成的RNA一样，编码序列包含基因内控制区。体外合成的两种RNA的相对丰度与体内观察到的不同。
