King W, Van Santen V, Kieff E
J Virol. 1981 May;38(2):649-60. doi: 10.1128/JVI.38.2.649-660.1981.
Nuclear and polyadenylated RNA fractions of Raji cells are encoded by larger fractions of Epstein-Barr virus DNA (35 and 18%, respectively) than encode polyribosomal RNA (10%). Polyribosomal RNA is encoded by DNA mapping at 0.05 X 10(8) to 0.29 X 10(8), 0.63 X 10(8) to 0.66 X 10(8), and 1.10 X 10(8) to 0.03 X 10(8) daltons. An abundant, small (160-base), non-polyadenylated RNA encoded by EcoRI fragment J (0.05 X 10(8) to 0.07 X 10(8) daltons) is also present in the cytoplasm of Raji cells. After induction of early antigen in Raji cells, there was a substantial increase in the complexity of viral polyadenylated and polyribosomal RNAs. Thus, nuclear RNA was encoded by 40% of Epstein-Barr virus DNA, and polyadenylated and polyribosomal RNAs were encoded by at least 30% of Epstein-Barr virus DNA. Polyribosomal RNA from induced Raji cells was encoded by Epstein-Barr virus DNAs mapping at 0.05 X 10(8) to 0.29 X 10(8), 0.63 X 10(8) to 0.66 X 10(8), and 1.10 X 10(8) to 0.03 X 10(8) daltons and also by DNAs mapping within the long unique regions of Epstein-Barr virus DNA at 0.39 X 10(8) to 0.49 X 10(8), 0.51 X 10(8) to 0.59 X 10(8), 0.66 X 10(8) to 0.77 X 10(8), and 1.02 X 10(8) to 1.05 X 10(8) daltons.
拉吉细胞的核RNA和多聚腺苷酸化RNA组分由较大比例的爱泼斯坦-巴尔病毒DNA编码（分别为35%和18%），高于编码多核糖体RNA的比例（10%）。多核糖体RNA由定位在0.05×10⁸至0.29×10⁸、0.63×10⁸至0.66×10⁸以及1.10×10⁸至0.03×10⁸道尔顿的DNA编码。由EcoRI片段J（0.05×10⁸至0.07×10⁸道尔顿）编码的一种丰富的小（160个碱基）、非多聚腺苷酸化RNA也存在于拉吉细胞的细胞质中。在拉吉细胞中诱导早期抗原后，病毒多聚腺苷酸化RNA和多核糖体RNA的复杂性显著增加。因此，核RNA由40%的爱泼斯坦-巴尔病毒DNA编码，多聚腺苷酸化RNA和多核糖体RNA由至少30%的爱泼斯坦-巴尔病毒DNA编码。来自诱导拉吉细胞的多核糖体RNA由定位在0.05×10⁸至0.29×10⁸、0.63×10⁸至0.66×10⁸以及1.10×10⁸至0.03×10⁸道尔顿的爱泼斯坦-巴尔病毒DNA编码，也由定位在爱泼斯坦-巴尔病毒DNA长独特区域内、0.39×10⁸至0.49×10⁸、0.51×10⁸至0.59×10⁸、0.66×10⁸至0.77×10⁸以及1.02×10⁸至1.05×10⁸道尔顿的DNA编码。
