Hayashi J, Gotoh O, Tagashira Y, Tosu M, Sekiguchi T, Yoshida M C
Somatic Cell Genet. 1982 Jan;8(1):67-81. doi: 10.1007/BF01538651.
Several mouse-rat somatic hybrid cell lines were isolated by fusing chloramphenicol-resistant (CAPr) and CAP-sensitive (CAPs) parent cells, and propagation of the parent mitochondrial DNA (mtDNA) species in the hybrid cells was studied. The restriction endonucleases EcoRI, HpaII, and HaeIII were used for identification of mtDNA species. Both mouse and rat mtDNAs were propagated in all the hybrid cells examined and maintained during long-term cultivation and repeated cell division. Moreover, in CAPr mouse-rat hybrid cells, selection and successive cultivation in the presence of CAP did not increase the relative amount of mtDNA species of CAPr parent cell origin, and when CAP was removed from the culture medium, mtDNA species of CAPr parent cell origin did not decrease appreciably. The amount of mouse mtDNAs was consistently 1-4 times that of rat mtDNAs inthe mouse-rat hybrid cells regardless of the species of parent cells from which the CAP resistance was derived. Thus mouse-rat hybrid cells have a stable mtDNA population in which the amount of mouse mtDNAs is larger than that of rat mtDNAs without any influence of CAP selection.
通过融合氯霉素抗性（CAPr）和亲本细胞的氯霉素敏感性（CAPs）分离出几种小鼠 - 大鼠体细胞杂种细胞系，并研究了杂种细胞中亲本线粒体DNA（mtDNA）种类的增殖情况。使用限制性内切酶EcoRI、HpaII和HaeIII来鉴定mtDNA种类。小鼠和大鼠的mtDNA在所有检测的杂种细胞中均能增殖，并在长期培养和反复细胞分裂过程中得以维持。此外，在CAPr小鼠 - 大鼠杂种细胞中，在CAP存在下进行选择和连续培养并不会增加CAPr亲本细胞来源的mtDNA种类的相对量，并且当从培养基中去除CAP时，CAPr亲本细胞来源的mtDNA种类也不会明显减少。无论CAP抗性源自何种亲本细胞，在小鼠 - 大鼠杂种细胞中，小鼠mtDNA的量始终是大鼠mtDNA量的1 - 4倍。因此，小鼠 - 大鼠杂种细胞具有稳定的mtDNA群体，其中小鼠mtDNA的量大于大鼠mtDNA的量，且不受CAP选择的任何影响。
