Hara H, Miyao M, Moriki T, Kutsukake F, Yamane T
Acta Pathol Jpn. 1982 May;32(3):385-98. doi: 10.1111/j.1440-1827.1982.tb01396.x.
Histologic and ultrastructural features of nephroblastomas in Sprague-Dawley rats induced by a single transplacental injection of ethylnitrosourea (ENU) on the 15-16th day of gestation are described and their significance is discussed with respect to the histogenesis of the tumors. Many renal tumors were observed, in addition to predominant occurrence of tumors of the nervous system. Forty-two of 142 offsprings survived over 100 days developed renal tumors, of which 37 rats had nephroblastomas, three rats had cortical adenomas, and one rat had a cystadenoma. The nephroblastomas were found in 35 rats unilaterally and in two rats bilaterally. Most of these tumors had various amounts of hyalinized stroma. In the kidneys with nephroblastomas, isolated, small foci of renal blastema were also occasionally noted. Electron microscopy revealed that nephroblastomas consisted of two types of cells, namely, epithelial and mesenchymal cells. The epithelial cells showed cellular differentiation of varying degree; they are freely lying in the stroma, forming well differentiated tubules or immature tubule-like structures and solid cell nests. Most of the undifferentiated cells observed around the tubular structures and cell nests are demonstrated to have epithelial features. These observations seem to support the concept that nephroblastoma is originated from metanephric blastema.
描述了在妊娠第15 - 16天经胎盘单次注射乙基亚硝基脲（ENU）诱导的Sprague-Dawley大鼠肾母细胞瘤的组织学和超微结构特征，并就肿瘤的组织发生讨论了其意义。除了主要发生神经系统肿瘤外，还观察到许多肾肿瘤。142只存活超过100天的后代中有42只发生了肾肿瘤，其中37只大鼠患有肾母细胞瘤，3只大鼠患有皮质腺瘤，1只大鼠患有囊腺瘤。35只大鼠的肾母细胞瘤为单侧发生，2只大鼠为双侧发生。这些肿瘤大多有不同程度的玻璃样变间质。在患有肾母细胞瘤的肾脏中，偶尔也会发现孤立的、小灶性的肾胚基。电子显微镜显示肾母细胞瘤由两种类型的细胞组成，即上皮细胞和间充质细胞。上皮细胞表现出不同程度的细胞分化；它们自由地位于间质中，形成分化良好的小管或不成熟的小管样结构以及实性细胞巢。在管状结构和细胞巢周围观察到的大多数未分化细胞被证明具有上皮特征。这些观察结果似乎支持肾母细胞瘤起源于后肾胚基的概念。
