Field H J, Larder B A, Darby G
Am J Med. 1982 Jul 20;73(1A):369-71. doi: 10.1016/0002-9343(82)90124-3.
A number of clinical studies have documented herpes simplex infections which appear to be resistant to nucleoside analogs; these include idoxuridine [1,2] and acyclovir [3]. Few, if any of the viruses isolated from such patients have yet been thoroughly characterized. We have isolated a number of acyclovir-resistant mutants by selection for resistance in tissue culture. The study of the biochemical and biological properties of these mutants has given some insight into the likely nature of resistant clinical strains. We have devised a number of simple tests to allow classification of laboratory mutants. We also draw attention to some of the difficulties the clinical virologist may encounter when analyzing putative resistant virus isolated from treated patients.
多项临床研究记录了似乎对核苷类似物耐药的单纯疱疹感染；这些感染包括碘苷[1,2]和阿昔洛韦[3]。从这类患者中分离出的病毒，即便有也很少得到充分鉴定。我们通过在组织培养中选择耐药性，分离出了多个阿昔洛韦耐药突变体。对这些突变体的生化和生物学特性进行研究，使我们对耐药临床菌株的可能性质有了一些了解。我们设计了一些简单的试验，以便对实验室突变体进行分类。我们还提请注意临床病毒学家在分析从接受治疗的患者中分离出的假定耐药病毒时可能遇到的一些困难。
