Vorbrodt A W, Lossinsky A S, Wisniewski H M
Brain Res. 1982 Jul 15;243(2):225-34. doi: 10.1016/0006-8993(82)90245-1.
Enzyme activity, representing the sites of K+-dependent p-nitrophenylphosphatase (K+-pNPPase), a component of the transport adenosine triphosphatase (Na+,K+-ATPase) system, has been localized at the ultrastructural in both cerebral cortex and in sciatic nerve of the mouse. Normal mice and animals with mechanically injured blood-brain barrier (BBB) were used. In the cerebral cortex, positive reaction was found in synapses, plasmalemma of neurites (axons and dendrites), in endothelial cell of microblood vessels and in the plasmalemma of mesothelial cells of the pia mater. In the sciatic nerve, a strong reaction was present in the nodes of Ranvier, with weaker reaction in the internodal areas of the axolemma. In the endothelial cells of normal blood vessels, the reaction product was localized on the luminal and abluminal, or only on the abluminal plasmalemma. After damage of BBB, numerous invaginations, pits and pinocytic vesicles showing positive reaction in their limiting membrane appeared in the endothelial cells.
酶活性代表了钾离子依赖性对硝基苯磷酸酶（K⁺-pNPPase）的位点，它是转运三磷酸腺苷酶（Na⁺,K⁺-ATPase）系统的一个组成部分，已在小鼠大脑皮层和坐骨神经的超微结构中定位。使用了正常小鼠和血脑屏障（BBB）受到机械损伤的动物。在大脑皮层中，在突触、神经突（轴突和树突）的质膜、微血管内皮细胞和软脑膜间皮细胞的质膜中发现了阳性反应。在坐骨神经中，郎飞结处有强烈反应，轴膜节间区域反应较弱。在正常血管的内皮细胞中，反应产物定位于管腔面和管腔外表面，或仅定位于管腔外质膜。血脑屏障受损后，内皮细胞中出现大量内陷、小窝和有被小泡，其限制膜呈阳性反应。
