鸡胚十二指肠细胞质中1,25(OH)₂D₃受体的生化特性
Biochemical characterization of 1,25(OH)2D3 receptors in chick embryonal duodenal cytosol.
作者信息
Seino Y, Yamaoka K, Ishida M, Yabuuchi H, Ichikawa M, Ishige H, Yoshino H, Avioli L V
出版信息
Calcif Tissue Int. 1982 May;34(3):265-9. doi: 10.1007/BF02411248.
This study presents measurements of serum vitamin D metabolites, calcium and phosphorus as well as measurements of the equilibrium dissociation constant for duodenal 1,25(OH)2D3 receptor in 15-, 18-, 19-, and 20-day chick embryos in comparison to that in 1- and 118-day-old chicks and to vitamin D-deficient chicks. The present results showed that: (a) serum 1,25(OH)2D and 24,25(OH)2D levels rise from 15 and 18 to days 19 and 20 of embryonic development while serum phosphate levels are stable; (b) serum calcium levels rise at hatching to adult levels; (c) the duodenal 1,25(OH)2D3 receptor is detectable in 15-day-old embryo and has a Kd similar to that of 118-day-old vitamin D-replete chicks; and (d) the activity of 1,25(OH)2D3 receptor in chick duodenal cytosol is maximal at hatching.
本研究呈现了15日龄、18日龄、19日龄和20日龄鸡胚血清维生素D代谢产物、钙和磷的测量结果，以及十二指肠1,25(OH)₂D₃受体平衡解离常数的测量结果，并与1日龄和118日龄雏鸡以及维生素D缺乏雏鸡进行了比较。目前的结果表明：(a)血清1,25(OH)₂D和24,25(OH)₂D水平在胚胎发育的第15天和18天到第19天和20天升高，而血清磷酸盐水平稳定；(b)血清钙水平在孵化时升至成年水平；(c)十二指肠1,25(OH)₂D₃受体在15日龄胚胎中可检测到，其解离常数(Kd)与118日龄维生素D充足雏鸡相似；(d)鸡十二指肠细胞质中1,25(OH)₂D₃受体的活性在孵化时最大。
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