DL-乙硫氨酸对大鼠肝脏中NAD含量及聚（ADP-核糖）合成酶活性的影响。

Influence of DL-ethionine on the NAD content and on the activity of the poly(ADPR)synthetase in the rat liver.

Kröger H, Grätz R, Grahn H

Int J Biochem. 1982;14(7):557-60. doi: 10.1016/0020-711x(82)90034-9.

Abstract

Ethionine, an analogue of methionine, is known to cause a number of serious changes. In this study, its influence on the NAD + NADH2 content and on the activity of the poly(ADPR)synthetase in rat liver has been investigated. 2. DL-Ethionine decreases the content of NAD + NADH2 in adrenalectomized, but not in normal animals. 3. After injection of nicotinamide the NAD + NADH2 content in the liver of adrenalectomized rats is much higher than in that of normal ones. 4. DL-Ethionine diminishes the NAD synthesis caused by nicotinamide in adrenalectomized animals and increases it in normal ones. DL-Tryptophan induces only a slight NAD synthesis, which is reduced in adrenalectomized animals by thionine. 5. 1-Methylnicotinamide slightly decreases the NAD concentration in normal animals, but increases it considerably in adrenalectomized ones. DL-ethionine reduces the NAD + NADH content in adrenalectomized animals in the presence of 1-methylnicotinamide. 6. The activity of poly(ADPR)synthetase of in-vitro tested nuclei is slightly increased after treatment with DL-ethionine.

摘要

乙硫氨酸是蛋氨酸的类似物，已知会引起许多严重变化。在本研究中，已对其对大鼠肝脏中NAD + NADH2含量和聚（ADPR）合成酶活性的影响进行了研究。2. DL-乙硫氨酸会降低肾上腺切除动物肝脏中NAD + NADH2的含量，但对正常动物则无此影响。3. 注射烟酰胺后，肾上腺切除大鼠肝脏中的NAD + NADH2含量远高于正常大鼠。4. DL-乙硫氨酸会减少肾上腺切除动物中由烟酰胺引起的NAD合成，而在正常动物中则会增加。DL-色氨酸仅诱导轻微的NAD合成，而在肾上腺切除动物中，硫氨酸会降低这种合成。5. 1-甲基烟酰胺会使正常动物中的NAD浓度略有降低，但会使肾上腺切除动物中的NAD浓度大幅增加。在存在1-甲基烟酰胺的情况下，DL-乙硫氨酸会降低肾上腺切除动物中NAD + NADH的含量。6. 用DL-乙硫氨酸处理后，体外测试的细胞核中聚（ADPR）合成酶的活性略有增加。