AKR嗜异性小鼠白血病病毒未整合前病毒DNA的分子克隆

Molecular cloning of AKR xenotropic murine leukemia virus unintegrated proviral DNA.

作者信息

Joseph D R

出版信息

Gene. 1982 Mar;17(3):341-4. doi: 10.1016/0378-1119(82)90151-2.

Abstract

Suprahelical proviral DNA of AKR xenotropic murine leukemia virus was purified from agarose gels and cloned in lambda Charon 28 DNA (BamHI sites). Nine viral DNA recombinants were identified and mapped with 12 restriction endonucleases. Three calsses of cloned viral DNA inserts were found: (1) Six inserts were apparently full-length 9.0-kb DNA with tandem long terminal repeat (LTR) elements; (2) two inserts contained DNAs with deletions in or adjacent to the LTR regions; (3) a single isolate contained an inversion of 2.3 kb around the LTR in the envelope gene.

摘要

从琼脂糖凝胶中纯化出AKR嗜异性鼠白血病病毒的超螺旋前病毒DNA，并克隆到λ噬菌体Charon 28 DNA（BamHI位点）中。鉴定出9个病毒DNA重组体，并用12种限制性内切核酸酶进行了图谱分析。发现了三类克隆的病毒DNA插入片段：（1）六个插入片段显然是全长9.0 kb的DNA，带有串联的长末端重复（LTR）元件；（2）两个插入片段包含在LTR区域内或其附近有缺失的DNA；（3）一个单独的分离株在包膜基因的LTR周围有2.3 kb的倒位。