Lowy D R, Rands E, Chattopadhyay S K, Garon C F, Hager G L
Proc Natl Acad Sci U S A. 1980 Jan;77(1):614-8. doi: 10.1073/pnas.77.1.614.
The lack of an endonuclease EcoRI site in the AKR murine leukemia virus (MuLV) DNA genome was utilized to molecularly clone, in Charon 4A lambda DNA, integrated infectious AKR MuLV DNA isolated from productively infected mouse cells. Three lambda-mouse recombinants (clones 614, 621, and 623) were selected by virtue of their reactivity with AKR MuLV [32P]cDNA. Clones 614 and 623 contained the complete AKR MuLV DNA flanked by nonviral cell sequences of which no more than 100 base pairs beyond the viral DNA appear to be shared. DNAs from both clones 614 and 623 were highly infectious for mouse cells and yielded N-tropic ecotropic MuLV; the specific infectivity of the DNA and the titer of the derived virus was more than 10-fold higher with 623. Clone 621 contained only some viral DNA and was not infectious under similar conditions.
利用艾氏鼠白血病病毒（MuLV）DNA基因组中缺乏核酸内切酶EcoRI位点这一特性，将从产生感染性病毒的小鼠细胞中分离得到的整合型感染性AKR MuLV DNA，在Charon 4A λ噬菌体DNA中进行分子克隆。通过它们与AKR MuLV [32P]cDNA的反应性，筛选出三个λ-小鼠重组体（克隆614、621和623）。克隆614和623含有完整的AKR MuLV DNA，两侧是非病毒细胞序列，病毒DNA两侧不超过100个碱基对的序列似乎是共享的。克隆614和623的DNA对小鼠细胞具有高度感染性，并产生N-嗜性亲嗜性MuLV；克隆623的DNA的比感染性和衍生病毒的滴度比克隆614高10倍以上。克隆621仅包含一些病毒DNA，在类似条件下无感染性。
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