Crabtree G R, Munck A, Smith K A
J Immunol. 1980 May;124(5):2430-5.
Recently a 2- to 3-fold increase in the number of glucocorticoid receptors in human peripheral lymphocytes has been noted after in vitro mitogen stimulation. Here, we extend these observations to in vivo immunization. After unilateral immunization of adrenalectomized male rats, a 50% increase in glucocorticoid receptor sites per cell, determined by binding of dexamethasone, was observed in cell suspensions of homolateral lymph nodes over those from the contralateral nonimmunized side of the same animal. The association constant for dexamethasone was similar in both groups, as was the stereospecificity for various steroids, the time course of cytoplasmic and nuclear association, and cytoplasmic-to-nuclear translocation. Despite a 50% increase in the number of glucocorticoid receptor sites per cell, the cells from the homolateral and controlateral lymph nodes were equally sensitive to the inhibitory effects of dexamethasone, as determined by measurements of the incorporation of radiolabeled precursors of protein, RNA, and DNA, or measurements of in vitro cell survival.
最近发现，体外有丝分裂原刺激后人外周淋巴细胞中糖皮质激素受体数量增加了2至3倍。在此，我们将这些观察结果扩展至体内免疫。在对肾上腺切除的雄性大鼠进行单侧免疫后，通过地塞米松结合测定发现，同侧淋巴结细胞悬液中每细胞糖皮质激素受体位点数量比同一动物对侧未免疫侧增加了50%。两组地塞米松的结合常数相似，各种类固醇的立体特异性、细胞质和细胞核结合的时间进程以及细胞质到细胞核的转运也相似。尽管每细胞糖皮质激素受体位点数量增加了50%，但通过测量放射性标记的蛋白质、RNA和DNA前体的掺入量或体外细胞存活率测定发现，同侧和对侧淋巴结的细胞对地塞米松的抑制作用同样敏感。
