Gluck S, Kelly S, Al-Awqati Q
J Biol Chem. 1982 Aug 25;257(16):9230-3.
H+ secretion by the turtle urinary bladder is produced by a proton pump located in the luminal membrane. We show that a microsomal fraction of these cells contains an electrogenic proton translocating ATPase that is inhibited by dicyclohexylcarbodiimide but not oligomycin or vanadate. On a sucrose density gradient, this ATPase co-migrated with luminal membranes labeled with concanavalin A, but was separate from a lysosomal marker. This enzyme is therefore the H+ ATPase that causes urinary acidification.
乌龟膀胱分泌H⁺是由位于管腔膜上的质子泵产生的。我们发现这些细胞的微粒体部分含有一种生电质子转运ATP酶，它被二环己基碳二亚胺抑制，但不受寡霉素或钒酸盐抑制。在蔗糖密度梯度上，这种ATP酶与用伴刀豆球蛋白A标记的管腔膜共同迁移，但与溶酶体标记物分开。因此，这种酶就是导致尿液酸化的H⁺ATP酶。
