Bennett H P, Browne C A, Solomon S
J Biol Chem. 1982 Sep 10;257(17):10096-102.
The isolation and characterization of eight forms of corticotropin-like intermediary lobe peptide (CLIP, adrenocorticotropin18-39) from the intermediary lobe of the rat pituitary has been accomplished by using reversed phase high performance liquid chromatography. The eight forms are the result of all combinations of the presence or absence of three post-translational modifications. These are glycosylation, phosphorylation, and removal of the carboxyl-terminal amino acid. The sites of phosphorylation and glycosylation are at serine 31 and asparagine 29, respectively. The eight forms (in order of elution from the reversed high performance liquid chromatography column) are glycosylated, phosphorylated CLIP18-38; glycosylated, nonphosphorylated CLIP18-38; nonglycosylated, phosphorylated CLIP18-38; nonglycosylated, nonphosphorylated CLIP18-38; glycosylated, phosphorylated CLIP18-39; glycosylated, nonphosphorylated CLIP18-39; nonglycosylated, phosphorylated CLIP18-39; and nonglycosylated, nonphosphorylated CLIP18-39.
通过反相高效液相色谱法，已从大鼠脑垂体中间叶分离并鉴定出八种促肾上腺皮质激素样中叶肽（CLIP，促肾上腺皮质激素18 - 39）。这八种形式是三种翻译后修饰存在或不存在的所有组合的结果。这些修饰是糖基化、磷酸化以及羧基末端氨基酸的去除。磷酸化和糖基化位点分别在丝氨酸31和天冬酰胺29处。这八种形式（按从反相高效液相色谱柱洗脱的顺序）是糖基化、磷酸化的CLIP18 - 38；糖基化、非磷酸化的CLIP18 - 38；非糖基化、磷酸化的CLIP18 - 38；非糖基化、非磷酸化的CLIP18 - 38；糖基化、磷酸化的CLIP18 - 39；糖基化、非磷酸化的CLIP18 - 39；非糖基化、磷酸化的CLIP18 - 39；以及非糖基化、非磷酸化的CLIP18 - 39。
