Ewert D L, Halpern M S
J Immunol. 1982 Oct;129(4):1724-30.
Plasma cells of line 15I5 chickens have been shown to express antigen that is cross-reactive with endogenous retroviral envelope glycoprotein. In the present study, we isolated this antigen and showed that it is structurally homologous to envelope glycoprotein. As assayed by immunofluorescence, most, if not all, of the plasma cell-associated envelope glycoprotein is present on the cell surface although it is not bound to surface Ig or Ia antigen. Although envelope antigen was not detectable by immunofluorescence on the surface of 15I5 bursal cells or peripheral B lymphocytes, immunochemical assays established that retroviral envelope glycoprotein is present on the surface of the bursal cells but at much lower levels than on the surface of the plasma cells. Both bursal and plasma cells synthesize envelope glycoprotein. These findings indicate that an increased level of expression of endogenous retroviral envelope glycoprotein is a concomitant of the differentiation of B lymphocytes to plasma cells in 15I5 chickens.
15I5品系鸡的浆细胞已被证明可表达与内源性逆转录病毒包膜糖蛋白发生交叉反应的抗原。在本研究中，我们分离出了这种抗原，并表明它在结构上与包膜糖蛋白同源。通过免疫荧光检测，大部分（如果不是全部的话）与浆细胞相关的包膜糖蛋白存在于细胞表面，尽管它不与表面免疫球蛋白或Ia抗原结合。虽然在15I5法氏囊细胞或外周B淋巴细胞表面通过免疫荧光无法检测到包膜抗原，但免疫化学分析确定逆转录病毒包膜糖蛋白存在于法氏囊细胞表面，但其水平比浆细胞表面低得多。法氏囊细胞和浆细胞都能合成包膜糖蛋白。这些发现表明，内源性逆转录病毒包膜糖蛋白表达水平的升高是15I5品系鸡B淋巴细胞向浆细胞分化的一个伴随现象。
