Halpern M S, Mason W S, Ewert D L, Deery D T, England J M
J Immunol. 1981 Mar;126(3):1198-202.
In a previous study (1), we observed that antigen cross-reactive with structural protein of the endogenous retrovirus RAV-O is expressed in splenic Ig(+) B lymphocytes of immunized 15I5 X 71 chickens. The present experiments were undertaken to determine the relationship of this antigen to viral gag- and env-coded antigenic specificities. As analyzed by immunofluorescence with several antisera, group-specific determinants of the gp85 envelope glycoprotein as well as viral-related type E-specific determinants were detectable in islands of Ig(+) splenocytes; antigenic determinants of viral gag protein were not detected in the splenic islands. These observations indicated that antigen cross-reactive with endogenous retroviral envelope glycoprotein is expressed in Ig(+) B cells.
在之前的一项研究（1）中，我们观察到与内源性逆转录病毒RAV - O的结构蛋白发生交叉反应的抗原，在免疫后的15I5 X 71鸡的脾脏Ig(+) B淋巴细胞中表达。开展本实验以确定该抗原与病毒gag和env编码的抗原特异性之间的关系。通过用几种抗血清进行免疫荧光分析，在Ig(+)脾细胞岛中可检测到gp85包膜糖蛋白的群特异性决定簇以及病毒相关的E型特异性决定簇；在脾脏岛中未检测到病毒gag蛋白的抗原决定簇。这些观察结果表明，与内源性逆转录病毒包膜糖蛋白发生交叉反应的抗原在Ig(+) B细胞中表达。
