浆细胞中的内源性逆转录病毒包膜抗原。

Endogenous retroviral envelope antigen in plasma cells.

作者信息

Halpern M S, Ewert D L, Flores L J, Lin K Y, England J M

出版信息

J Immunol. 1981 Aug;127(2):698-702.

PMID:7019329

Abstract

Previous observations that endogenous retroviral envelope antigen is expressed in splenic Ig(+) B cells of SRBC-sensitized 15I5 X 71 chickens suggested that expression of this antigen might be a general property of plasma cells in 15I5 and related strains. To investigate this possibility, immunofluorescence assays with antiviral sera were carried out using plasma cells present in Harder glands. Endogenous retroviral envelope antigen was detectable in plasma cells of line 15I5 X 71, 15I5 X 72, and 15I5 chickens but not in plasma cells of line 72, 15B, or 63 chickens. Higher levels of retroviral envelope antigen were detectable in 15I5 X 71 plasma cells as compared to 15I5 X 71 bursal cells. The presence of retroviral envelope antigen in 15I5 plasma cells, coupled with the absence of detectable levels of retroviral p27-related antigen in these cells, suggested that expression of the former is governed by the ev 6 genetic locus.

摘要

先前的观察结果表明，内源性逆转录病毒包膜抗原在经绵羊红细胞致敏的15I5×71品系鸡的脾脏Ig(+) B细胞中表达，这表明该抗原的表达可能是15I5及相关品系浆细胞的普遍特性。为了研究这种可能性，使用哈德氏腺中存在的浆细胞，对抗病毒血清进行了免疫荧光测定。在15I5×71、15I5×72和15I5品系鸡的浆细胞中可检测到内源性逆转录病毒包膜抗原，但在72、15B或63品系鸡的浆细胞中未检测到。与15I5×71法氏囊细胞相比，在15I5×71浆细胞中可检测到更高水平的逆转录病毒包膜抗原。15I5浆细胞中存在逆转录病毒包膜抗原，而这些细胞中未检测到可检测水平的逆转录病毒p27相关抗原，这表明前者的表达受ev 6基因座控制。