博尔顿-亨特标记的胆囊收缩素-33与羧基末端胆囊收缩素抗体的低结合力。
Low binding of Bolton-Hunter-labelled cholecystokinin-33 to carboxyl-terminal CCK-antibodies.
作者信息
Jansen J B, Lamers C B
出版信息
J Immunol Methods. 1982 Jun 11;51(2):223-30. doi: 10.1016/0022-1759(82)90261-7.
PMID:6286777
Abstract
Labelling of cholecystokinin-33 (CCK33) with [125I]hydroxyphenylpropionic acid-succinimide ester (Bolton-Hunter-reagent) results in labels with excellent immunoreactivity when tested with non-carboxyl-terminal CCK-antibodies but extremely low binding to carboxyl-terminal CCK-antibodies. This finding might explain some of the difficulties experienced with production of immunoreactive CCK33 labels prepared by the Bolton-Hunter method.
摘要
用[125I]羟苯基丙酸琥珀酰亚胺酯（博尔顿-亨特试剂）标记胆囊收缩素-33（CCK33），当用非羧基末端CCK抗体检测时，标记物具有优异的免疫反应性，但与羧基末端CCK抗体的结合极低。这一发现可能解释了用博尔顿-亨特方法制备免疫反应性CCK33标记物时遇到的一些困难。
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