• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

腺病毒DNA结合蛋白可刺激由腺病毒和腺相关病毒启动子指导的转录速率。

The adenovirus DNA-binding protein stimulates the rate of transcription directed by adenovirus and adeno-associated virus promoters.

作者信息

Chang L S, Shenk T

机构信息

Department of Biology, Howard Hughes Medical Institute, Princeton University, New Jersey 08544.

出版信息

J Virol. 1990 May;64(5):2103-9. doi: 10.1128/JVI.64.5.2103-2109.1990.

DOI:10.1128/JVI.64.5.2103-2109.1990
PMID:2157873
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC249367/
Abstract

The DNA-binding protein (DBP) encoded by the E2A region of adenovirus type 5 was found to enhance the expression of a reporter gene controlled by several different promoters within transfected cells. The rate of synthesis of correctly initiated transcripts was increased by the DBP. The adeno-associated virus P5 promoter and the adenovirus E1A and E2A early and major late promoters responded to the DBP by increases in expression ranging from 6- to 27-fold, while the adenovirus E4 promoter was slightly inhibited by DBP. The adenovirus major late promoter showed a greater response to DBP than to the E1A transactivator protein, suggesting that the DBP plays a central role in activation of the late promoter.

摘要

人们发现，由5型腺病毒E2A区域编码的DNA结合蛋白（DBP）可增强转染细胞内由几种不同启动子控制的报告基因的表达。DBP提高了正确起始转录本的合成速率。腺相关病毒P5启动子以及腺病毒E1A和E2A早期及主要晚期启动子对DBP有反应，表达增加6至27倍，而腺病毒E4启动子受到DBP的轻微抑制。腺病毒主要晚期启动子对DBP的反应比对E1A反式激活蛋白的反应更大，这表明DBP在晚期启动子的激活中起核心作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9246/249367/b5b9b0ec822a/jvirol00060-0242-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9246/249367/8656e15f40fa/jvirol00060-0240-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9246/249367/a8d8c42b4e73/jvirol00060-0241-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9246/249367/b5b9b0ec822a/jvirol00060-0242-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9246/249367/8656e15f40fa/jvirol00060-0240-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9246/249367/a8d8c42b4e73/jvirol00060-0241-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9246/249367/b5b9b0ec822a/jvirol00060-0242-a.jpg

相似文献

1
The adenovirus DNA-binding protein stimulates the rate of transcription directed by adenovirus and adeno-associated virus promoters.腺病毒DNA结合蛋白可刺激由腺病毒和腺相关病毒启动子指导的转录速率。
J Virol. 1990 May;64(5):2103-9. doi: 10.1128/JVI.64.5.2103-2109.1990.
2
Adenovirus early region 3 promoter regulation by E1A/E1B is independent of alterations in DNA binding and gene activation of CREB/ATF and AP1.腺病毒早期区域3启动子受E1A/E1B的调控独立于CREB/ATF和AP1的DNA结合及基因激活的改变。
J Virol. 1990 May;64(5):2004-13. doi: 10.1128/JVI.64.5.2004-2013.1990.
3
The adenovirus E1B 19-kilodalton protein stimulates gene expression by increasing DNA levels.腺病毒E1B 19千道尔顿蛋白通过增加DNA水平来刺激基因表达。
Mol Cell Biol. 1989 Dec;9(12):5412-23. doi: 10.1128/mcb.9.12.5412-5423.1989.
4
The role of the two E1a mRNA products of subgroup B adenoviruses in the regulation of early promoters of subgroup C adenoviruses.B 亚组腺病毒的两种 E1a mRNA 产物在 C 亚组腺病毒早期启动子调控中的作用。
Gene. 1988 Sep 15;69(1):111-20. doi: 10.1016/0378-1119(88)90383-6.
5
The adenovirus E1A transforming protein activates the proliferating cell nuclear antigen promoter via an activating transcription factor site.腺病毒E1A转化蛋白通过一个激活转录因子位点激活增殖细胞核抗原启动子。
J Virol. 1991 Dec;65(12):6397-406. doi: 10.1128/JVI.65.12.6397-6406.1991.
6
Two functions encoded by adenovirus early region 1A are responsible for the activation and repression of the DNA-binding protein gene.腺病毒早期区域1A编码的两种功能负责DNA结合蛋白基因的激活和抑制。
EMBO J. 1985 Mar;4(3):707-13. doi: 10.1002/j.1460-2075.1985.tb03687.x.
7
Inhibition of interferon-inducible gene expression by adenovirus E1A proteins: block in transcriptional complex formation.腺病毒E1A蛋白对干扰素诱导基因表达的抑制作用：转录复合物形成受阻。
Proc Natl Acad Sci U S A. 1991 Sep 1;88(17):7459-63. doi: 10.1073/pnas.88.17.7459.
8
Adeno-associated virus P5 promoter contains an adenovirus E1A-inducible element and a binding site for the major late transcription factor.腺相关病毒P5启动子包含一个腺病毒E1A诱导元件和一个主要晚期转录因子的结合位点。
J Virol. 1989 Aug;63(8):3479-88. doi: 10.1128/JVI.63.8.3479-3488.1989.
9
DNA octamer element can confer E1A trans-activation, and adenovirus infection results in a stimulation of the DNA-binding activity of OTF-1/NFIII factor.DNA八聚体元件可赋予E1A反式激活作用，腺病毒感染会刺激OTF-1/NFIII因子的DNA结合活性。
Proc Natl Acad Sci U S A. 1990 Aug;87(15):5878-82. doi: 10.1073/pnas.87.15.5878.
10
A cellular protein, activating transcription factor, activates transcription of multiple E1A-inducible adenovirus early promoters.一种细胞蛋白，即激活转录因子，可激活多种E1A诱导型腺病毒早期启动子的转录。
Proc Natl Acad Sci U S A. 1987 Dec;84(23):8355-9. doi: 10.1073/pnas.84.23.8355.

引用本文的文献

1
Advances of Recombinant Adenoviral Vectors in Preclinical and Clinical Applications.腺相关病毒载体在临床前和临床应用中的进展。
Viruses. 2024 Feb 28;16(3):377. doi: 10.3390/v16030377.
2
Acute Adenoviral Infection Elicits an Arrhythmogenic Substrate Prior to Myocarditis.急性腺病毒感染在心肌炎发生之前引发致心律失常基质。
Circ Res. 2024 Mar 29;134(7):892-912. doi: 10.1161/CIRCRESAHA.122.322437. Epub 2024 Feb 28.
3
Human Bocavirus 1 NP1 acts as an ssDNA-binding protein to help AAV2 DNA replication and cooperates with RPA to regulate AAV2 capsid expression.

本文引用的文献

1
Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.在哺乳动物细胞中表达氯霉素乙酰转移酶的重组基因组。
Mol Cell Biol. 1982 Sep;2(9):1044-51. doi: 10.1128/mcb.2.9.1044-1051.1982.
2
The primary transcription unit of the mouse beta-major globin gene.小鼠β-珠蛋白基因的初级转录单位。
Cell. 1981 Feb;23(2):585-93. doi: 10.1016/0092-8674(81)90154-9.
3
Regulation of early adenovirus transcription: a protein product of early region 2 specifically represses region 4 transcription.早期腺病毒转录的调控：早期区域2的一种蛋白质产物特异性抑制区域4的转录。
人博卡病毒 1 型 NP1 作为一种 ssDNA 结合蛋白，有助于 AAV2 DNA 的复制，并与 RPA 合作调节 AAV2 衣壳的表达。
J Virol. 2024 Mar 19;98(3):e0151523. doi: 10.1128/jvi.01515-23. Epub 2024 Feb 7.
4
The adenovirus DNA-binding protein DBP.腺病毒DNA结合蛋白DBP。
J Virol. 2024 Feb 20;98(2):e0188523. doi: 10.1128/jvi.01885-23. Epub 2024 Jan 10.
5
Fluorescence Microscopy in Adeno-Associated Virus Research.腺相关病毒研究中的荧光显微镜技术
Viruses. 2023 May 16;15(5):1174. doi: 10.3390/v15051174.
6
Global Transcriptome Analyses of Cellular and Viral mRNAs during HAdV-C5 Infection Highlight New Aspects of Viral mRNA Biogenesis and Cytoplasmic Viral mRNA Accumulations.HAdV-C5 感染过程中细胞和病毒 mRNA 的全球转录组分析突显了病毒 mRNA 生成和细胞质病毒 mRNA 积累的新方面。
Viruses. 2022 Nov 1;14(11):2428. doi: 10.3390/v14112428.
7
Adipocyte commitment of 3T3-L1 cells is required to support human adenovirus 36 productive replication concurrent with altered lipid and glucose metabolism.3T3-L1 细胞的脂肪细胞分化是支持人腺病毒 36 有效复制所必需的，同时伴有脂质和葡萄糖代谢的改变。
Front Cell Infect Microbiol. 2022 Sep 27;12:1016200. doi: 10.3389/fcimb.2022.1016200. eCollection 2022.
8
Targeting the Retinoblastoma/E2F repressive complex by CDK4/6 inhibitors amplifies oncolytic potency of an oncolytic adenovirus.靶向视网膜母细胞瘤/E2F 抑制复合物的 CDK4/6 抑制剂增强了溶瘤腺病毒的抗肿瘤效力。
Nat Commun. 2022 Aug 10;13(1):4689. doi: 10.1038/s41467-022-32087-5.
9
PML Alternative Splice Products Differentially Regulate HAdV Productive Infection.PML 可变剪接产物差异调节 HAdV 有效感染。
Microbiol Spectr. 2022 Aug 31;10(4):e0078522. doi: 10.1128/spectrum.00785-22. Epub 2022 Jun 14.
10
E1B-55K Is a Phosphorylation-Dependent Transcriptional and Posttranscriptional Regulator of Viral Gene Expression in Human Adenovirus C5 Infection.E1B-55K 是人类腺病毒 C5 感染中病毒基因表达的依赖于磷酸化的转录和转录后调节因子。
J Virol. 2022 Mar 9;96(5):e0206221. doi: 10.1128/jvi.02062-21. Epub 2022 Jan 12.
Proc Natl Acad Sci U S A. 1980 Apr;77(4):1893-7. doi: 10.1073/pnas.77.4.1893.
4
The stability of early adenovirus mRNA is controlled by the viral 72 kd DNA-binding protein.早期腺病毒信使核糖核酸的稳定性由病毒72千道尔顿DNA结合蛋白控制。
Cell. 1981 Nov;26(3 Pt 1):371-9. doi: 10.1016/0092-8674(81)90206-3.
5
The adenovirus type 5 E1A transcriptional control region contains a duplicated enhancer element.5型腺病毒E1A转录控制区包含一个重复的增强子元件。
Cell. 1983 Jul;33(3):695-703. doi: 10.1016/0092-8674(83)90012-0.
6
Isolation and characterization of full-length cDNA clones for human alpha-, beta-, and gamma-actin mRNAs: skeletal but not cytoplasmic actins have an amino-terminal cysteine that is subsequently removed.人α-、β-和γ-肌动蛋白mRNA全长cDNA克隆的分离与鉴定：骨骼肌肌动蛋白而非细胞质肌动蛋白具有一个随后会被去除的氨基端半胱氨酸。
Mol Cell Biol. 1983 May;3(5):787-95. doi: 10.1128/mcb.3.5.787-795.1983.
7
A mutation in the adenovirus type 5 DNA binding protein that fails to autoregulate the production of the DNA binding protein.
Virology. 1982 Oct 30;122(2):481-5. doi: 10.1016/0042-6822(82)90249-5.
8
Characterization of the immediate-early functions of pseudorabies virus.伪狂犬病病毒即刻早期功能的特征分析
Virology. 1983 Dec;131(2):437-54. doi: 10.1016/0042-6822(83)90510-x.
9
The role of adenovirus early region 1A in the regulation of early regions 2A and 1B expression.腺病毒早期区域1A在早期区域2A和1B表达调控中的作用。
Virology. 1983 Nov;131(1):49-58. doi: 10.1016/0042-6822(83)90532-9.
10
Host range temperature-conditional mutants in the adenovirus DNA binding protein are defective in the assembly of infectious virus.
Virology. 1983 Apr 15;126(1):228-39. doi: 10.1016/0042-6822(83)90474-9.