Hamer D H, Kaehler M, Leder P
Cell. 1980 Oct;21(3):697-708. doi: 10.1016/0092-8674(80)90433-x.
We have constructed two SV40 recombinants carrying a complete mouse alpha-globin gene with its presumptive promoter region. In one recombinant the globin gene can be transcribed either from its own promoter or from the adjacent viral late region promoter. In the other efficient globin expression should depend only upon the promoter carried by the chromosomal globin gene. We show that both viruses direct the synthesis of functional globin mRNA in infected monkey kidney cells and that this mRNA has a 5' terminus indistinguishable from that of authentic globin mRNA. These results suggest that the cloned globin gene contains a functional promoter that is accurately recognized in monkey kidney cells.
我们构建了两个携带完整小鼠α-珠蛋白基因及其推定启动子区域的SV40重组体。在一个重组体中，珠蛋白基因既可以从其自身的启动子转录，也可以从相邻的病毒晚期区域启动子转录。在另一个重组体中，有效的珠蛋白表达应该仅取决于染色体珠蛋白基因所携带的启动子。我们表明，两种病毒都能在感染的猴肾细胞中指导功能性珠蛋白mRNA的合成，并且这种mRNA的5'末端与真实珠蛋白mRNA的5'末端无法区分。这些结果表明，克隆的珠蛋白基因包含一个在猴肾细胞中能被准确识别的功能性启动子。
