与爱泼斯坦-巴尔病毒感染细胞相关的丝状结构。
Filamentous structures associated with Epstein-Barr virus-infected cells.
作者信息
Moore C L, Griffith J D, Shaw J E
出版信息
J Virol. 1982 Jul;43(1):305-13. doi: 10.1128/JVI.43.1.305-313.1982.
Abstract
After the onset of Epstein-Barr virus DNA and protein synthesis 10 h after superinfection of Raji cells (a cell line containing Epstein-Barr virus DNA but not producing virus), filamentous structures 25 nm in diameter and 0.2 to 1.4 micrometers in length could be detected in the cell cytoplasm by electron microscopy. These structures banded in metrizamide gradients with viral DNA and proteins, but at a density different from that of virions or nucleocapsids. These filaments, enriched in a 155,000-dalton protein similar in size to a major nucleocapsid protein of Epstein-Barr virus, may represent intermediates in viral nucleocapsid assembly.
摘要
在用爱泼斯坦-巴尔病毒超感染Raji细胞（一种含有爱泼斯坦-巴尔病毒DNA但不产生病毒的细胞系）10小时后，爱泼斯坦-巴尔病毒DNA和蛋白质合成开始，通过电子显微镜可在细胞质中检测到直径25纳米、长度0.2至1.4微米的丝状结构。这些结构在氯化铯密度梯度中与病毒DNA和蛋白质一起形成条带，但密度与病毒粒子或核衣壳不同。这些细丝富含一种大小与爱泼斯坦-巴尔病毒主要核衣壳蛋白相似的155,000道尔顿蛋白质，可能代表病毒核衣壳组装的中间体。
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