通过Raji细胞双重感染获得的爱泼斯坦-巴尔病毒的转化活性。
Transforming activity of Epstein-Barr virus obtained by superinfection of Raji cells.
作者信息
Yajima Y, Marczynska B, Nonoyama M
出版信息
Proc Natl Acad Sci U S A. 1978 Apr;75(4):2008-10. doi: 10.1073/pnas.75.4.2008.
Epstein-Barr virus obtained by superinfection of Raji cells with Epstein-Barr virus recovered from P3HR1 cells (HRI virus) transformed human lymphocytes, but it did not superinfect Raji cells. A human lymphoblastoid cell line, HLB, established by such transformation contained 22 Epstein-Barr virus genomes per cell and Epstein-Barr virus-associated nuclear antigen, and a few cells contained early or viral capsid antigen complexes. Chromosomal analysis revealed that HLB-cells were diploid with normal female karyotypes. Replication of Epstein-Barr virus DNA and inhibition of host cell DNA synthesis were observed in HLB cells after superinfection with HR1 virus.
通过用从P3HR1细胞中回收的爱泼斯坦-巴尔病毒（HRI病毒）对Raji细胞进行超感染而获得的爱泼斯坦-巴尔病毒转化了人淋巴细胞，但它并未对Raji细胞进行超感染。通过这种转化建立的人淋巴母细胞系HLB每个细胞含有22个爱泼斯坦-巴尔病毒基因组以及爱泼斯坦-巴尔病毒相关核抗原，并且少数细胞含有早期或病毒衣壳抗原复合物。染色体分析显示HLB细胞是具有正常女性核型的二倍体。在用HR1病毒超感染后，在HLB细胞中观察到了爱泼斯坦-巴尔病毒DNA的复制以及宿主细胞DNA合成的抑制。
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