Rhoades M
J Virol. 1982 Aug;43(2):566-73. doi: 10.1128/JVI.43.2.566-573.1982.
The locations of 103 cleavage sites, produced by 13 restriction endonucleases, were mapped on the DNA of bacteriophage T5. Single- and double-digest fragment sizes were determined by agarose gel electrophoresis, using restriction fragments of phi X174 DNA and lambda DNA as molecular weight standards. Map coordinates were determined by a computer-based least-squares procedures (J. Schroeder and F. Blattner, Gene [Amst] 4:167-174, 1978). The fragment sizes predicted by the final map are all within 2% of the measured values. Based on this analysis, T5st(+) DNA contains 121,300 base pairs (Mr, 80.3 X 10(6) and has a terminal repetition of 10,160 base pairs (Mr, 6.7 X 10(6)). Restriction endonuclease analysis after treatment with exonuclease III and a single-strand-specific endonuclease allowed precise localization of five of the natural single-chain interruptions in T5 DNA. Revised locations for several T5 deletions were also determined.
由13种限制性内切酶产生的103个切割位点的位置被绘制在噬菌体T5的DNA上。使用φX174 DNA和λDNA的限制性片段作为分子量标准，通过琼脂糖凝胶电泳确定单酶切和双酶切片段的大小。图谱坐标通过基于计算机的最小二乘法程序确定（J. Schroeder和F. Blattner，《基因》[阿姆斯特丹]4:167 - 174，1978年）。最终图谱预测的片段大小与测量值的差异均在2%以内。基于此分析，T5st(+) DNA含有121,300个碱基对（分子量，80.3×10⁶），并且有10,160个碱基对（分子量，6.7×10⁶）的末端重复序列。在用核酸外切酶III和单链特异性内切酶处理后进行的限制性内切酶分析，使得能够精确确定T5 DNA中五个天然单链中断的位置。还确定了几个T5缺失的修订位置。
