Daniels D L, de Wet J R, Blattner F R
J Virol. 1980 Jan;33(1):390-400. doi: 10.1128/JVI.33.1.390-400.1980.
A map of bacteriophage lambda was constructed, including accurate positions for all 41 cut sites made by 12 different restriction enzymes. Over 100 fragments from single, multiple, and partial enzyme digestions were measured versus standards that were calibrated with respect to DNA molecules of known sequence. The data were subjected to least-squares analysis to assign map coordinates. In no case did a fragment size predicted from the map differ from the measurement of the fragment by more than +/- 5%. This low error rate was consistent in all size ranges of fragments. The total length of lambda was calculated as 49,133 nucleotide pairs. This probably is accurate to within 500 base pairs.
构建了噬菌体λ的图谱，其中包括12种不同限制酶切割产生的所有41个切割位点的精确位置。针对经已知序列DNA分子校准的标准物，测量了来自单酶切、多酶切和部分酶切的100多个片段。对数据进行最小二乘法分析以确定图谱坐标。在任何情况下，根据图谱预测的片段大小与片段测量值的差异均不超过±5%。这种低误差率在所有片段大小范围内都是一致的。计算得出λ的总长度为49,133个核苷酸对。这可能精确到500个碱基对以内。
