Marsh R C, Hepburn M L
J Virol. 1981 Apr;38(1):104-14. doi: 10.1128/JVI.38.1.104-114.1981.
A complete map of the cleavage sites of restriction endonucleases BamHI, BglII, KpnI, PvuI, SalI, and XbaI was determined for the cytosine-containing DNA of a bacteriophage T4 alc mutant. The 56 sequence-specific sites were assigned map coordinates based on a least-squares analysis of measured fragment lengths. Altogether, the lengths of 118 fragments from single and double enzyme digestions were measured by electrophoresis of the fragments in agarose gels. DNA fragments of known sequence or DNA fragments calibrated with fragments of known sequence were used as standards. The greatest deviation between an experimentally measured fragment length and its computed map coordinates was 3.0%; the average deviation was 0.8%. The total length of the wild-type T4 genome was calculated to be 166,200 base pairs.
测定了噬菌体T4 alc突变体含胞嘧啶DNA上限制性内切酶BamHI、BglII、KpnI、PvuI、SalI和XbaI的切割位点完整图谱。根据对测量片段长度的最小二乘法分析，为这56个序列特异性位点指定了图谱坐标。总共通过琼脂糖凝胶电泳测量了单酶和双酶消化产生的118个片段的长度。已知序列的DNA片段或用已知序列片段校准的DNA片段用作标准。实验测量的片段长度与其计算出的图谱坐标之间的最大偏差为3.0%；平均偏差为0.8%。计算出野生型T4基因组的总长度为166,200个碱基对。
