噬菌体T5的新型缺失突变体
New deletion mutant of bacteriophage T5.
作者信息
Rhoades M, Schwartz J, Wahl J M
出版信息
J Virol. 1980 Nov;36(2):622-6. doi: 10.1128/JVI.36.2.622-626.1980.
Abstract
Deletion mutants of bacteriphage T5 can be identified by analyzing the DNA content of single plaques, after alkaline denaturation, by agarose gel electrophoresis. Use of this procedure has resulted in the isolation of a new mutant, designated del2, that lacks a 1.6% segment located 68 to 70% from the left end of T5 DNA. This segment occurs between the early and late gene regions of T5 DNA.
摘要
噬菌体T5的缺失突变体可通过碱性变性后，利用琼脂糖凝胶电泳分析单个噬菌斑的DNA含量来鉴定。采用该方法已分离出一种新的突变体，命名为del2，它缺失了T5 DNA左端68%至70%位置的一个1.6%的片段。该片段位于T5 DNA的早期和晚期基因区域之间。
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