小鼠膈神经肌肉接头处乙酰胆碱诱发电流的噪声分析

Noise analysis of acetylcholine induced current at neuromuscular junctions of the mouse diaphragm.

作者信息

Tanzi F, Taglietti V, Rovida S

出版信息

Neurosci Lett. 1982 May 28;30(2):153-6. doi: 10.1016/0304-3940(82)90288-9.

Abstract

Miniature end-plate currents (mepcs) and membrane noise elicited by acetylcholine (ACh) iontophoresis were investigated at neuromuscular junctions of the mouse diaphragm. All the experiments were performed at a holding potential of -70 mV at a temperature of 19 degrees C. The equilibrium potential of the ACh response was estimated to be near zero; the mepcs displayed a peak amplitude of 2.46 +/- 0.13 nA (mean +/- S.E.) and relaxed exponentially with a time constant to 1.63 +/- 0.11 msec. Single ACh-activated channels had a conductance of 26.5 +/- 1.5 pS and a mean life time of 1.69 +/0- 0.13 msec.

摘要

在小鼠膈肌的神经肌肉接头处，研究了由乙酰胆碱（ACh）离子电泳引发的微小终板电流（mepcs）和膜噪声。所有实验均在19摄氏度、-70 mV的钳制电位下进行。ACh反应的平衡电位估计接近零；mepcs的峰值幅度为2.46±0.13 nA（平均值±标准误），并以1.63±0.11毫秒的时间常数呈指数衰减。单个ACh激活通道的电导为26.5±1.5 pS，平均寿命为1.69±0.13毫秒。