Van der Ploeg L H, Liu A Y, Michels P A, De Lange T, Borst P, Majumder H K, Weber H, Veeneman G H, Van Boom J
Nucleic Acids Res. 1982 Jun 25;10(12):3591-604. doi: 10.1093/nar/10.12.3591.
The expression of the gene for variant surface glycoprotein (VSG) 118 in Trypanosoma brucei is activated by transposing a DNA segment containing the gene and 1-2 kb in front of it to an expression site elsewhere in the genome. By S1 nuclease protection and RNA blotting experiments we show here the presence of several minor transcripts in trypanosomes synthesizing VSG 118, one of which covers the entire transposed segment. Comparison of the sequence of the 5' terminal segment of VSG 118 messenger RNA (mRNA), determined by primed reverse transcription, and the corresponding region of the 118 VSG gene, shows that the 5' terminal 34 nucleotides of the mRNA are not encoded in the 118 VSG gene contiguous with the remainder of the mRNA. We conclude that synthesis of a VSG mRNA involves splicing of a much longer primary transcript, which may start outside the transposed segment.
布氏锥虫中变异表面糖蛋白（VSG）118基因的表达是通过将包含该基因及其前方1 - 2 kb的DNA片段转座到基因组其他位置的一个表达位点来激活的。通过S1核酸酶保护实验和RNA印迹实验，我们在此证明在合成VSG 118的锥虫中存在几种次要转录本，其中一种覆盖了整个转座片段。通过引发逆转录确定的VSG 118信使RNA（mRNA）5'末端片段的序列与118 VSG基因的相应区域进行比较，结果表明mRNA的5'末端34个核苷酸并非由与mRNA其余部分相邻的118 VSG基因编码。我们得出结论，VSG mRNA的合成涉及一个长得多的初级转录本的剪接，该初级转录本可能起始于转座片段之外。
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