锥虫中变异抗原的基因。

The genes for variant antigens in trypanosomes.

作者信息

Borst P, Frasch A C, Bernards A, Hoeijmakers J H, Van der Ploeg L H, Cross G A

出版信息

Am J Trop Med Hyg. 1980 Sep;29(5 Suppl):1033-6. doi: 10.4269/ajtmh.1980.29.1033.

DOI:10.4269/ajtmh.1980.29.1033

Abstract

We have studied the mechanism of antigenic variation by using DNA complementary to the messenger RNAs for four variant surface glycoproteins of Trypanosoma brucei. Pure complementary DNAs were obtained by cloning as recombinant DNA in Escherichia coli. Using these complementary DNAs as hybridization probes, we have analyzed the genes for these variant surface glycoproteins. The results provide new information on the origin and evolution of antigenic variation, and on the mechanism involved in switching from one antigenic type to another.

摘要

我们利用与布氏锥虫四种可变表面糖蛋白的信使核糖核酸互补的DNA，研究了抗原变异的机制。通过在大肠杆菌中作为重组DNA进行克隆，获得了纯互补DNA。利用这些互补DNA作为杂交探针，我们分析了这些可变表面糖蛋白的基因。这些结果为抗原变异的起源和进化以及从一种抗原类型转换到另一种抗原类型所涉及的机制提供了新的信息。