Gomez S, Queiroz L S
Q J Exp Physiol. 1982 Jul;67(3):495-506. doi: 10.1113/expphysiol.1982.sp002664.
Botulinum toxin (BoTx) was injected into the muscles of one leg in mice causing local paralysis. Black widow spider venom (b.w.s.v.) was then injected into the paralysed muscles 3 or 15 d later. In both groups b.w.s.v. destroyed the nerve terminals poisoned by BoTx. In the 15 d group axonal sprouts, which had former due to the block of neuromuscular transmission by BoTx, were also destroyed. Within a few days the motor nerve terminal regenerated and the muscles recovered from paralysis at a faster rate than after BoTx alone. Recovery seemed to begin earlier in muscles where axonal sprouting was already advanced when b.w.s.v. was injected. The normal pattern of innervation was re-established in both groups, which was in marked contrast with muscles after BoTx alone where numerous sprouts and many ectopic end-plates had formed.
将肉毒杆菌毒素（BoTx）注射到小鼠一条腿的肌肉中，导致局部麻痹。然后在3天或15天后将黑寡妇蜘蛛毒液（b.w.s.v.）注射到麻痹的肌肉中。在两组中，b.w.s.v. 都破坏了被BoTx毒害的神经末梢。在15天组中，由于BoTx阻断神经肌肉传递而先前形成的轴突发芽也被破坏。几天内，运动神经末梢再生，肌肉从麻痹中恢复的速度比仅注射BoTx后更快。当注射b.w.s.v.时，在轴突发芽已经进展的肌肉中，恢复似乎更早开始。两组均重新建立了正常的神经支配模式，这与仅注射BoTx后的肌肉形成了明显对比，后者形成了大量芽和许多异位终板。
