Saland L C, Mennin S P, Selinfreund R, Rasmussen P
Regul Pept. 1982 May;3(5-6):371-81. doi: 10.1016/0167-0115(82)90060-x.
Neurointermediate lobes from amphibians (Rana pipiens) were incubated in Medium 199 containing dopamine, beta-endorphin or dopamine plus beta-endorphin. Dopamine inhibited melanocyte-stimulating hormone (MSH) secretion as measured by bioassay in hypophysectomized frogs, an effect which was transiently reversed by beta-endorphin. The effects of endorphin were in turn partially suppressed by the opiate antagonist, naloxone hydrochloride. Cells treated with all three agents exhibited expanded rough endoplasmic reticulum and decreased secretory granule content, indicative of peptide release and new synthesis. Beta-Endorphin alone did not stimulate MSH secretion above control levels, and at one time period was seen to reduce MSH secretion. The findings indicate a complex interaction between beta-endorphin and dopamine directly upon MSH secretion at the level of the neurointermediate lobe.
将来自两栖动物（豹蛙）的神经中间叶置于含有多巴胺、β-内啡肽或多巴胺加β-内啡肽的199培养基中进行孵育。通过生物测定法检测，多巴胺可抑制垂体切除青蛙体内促黑素细胞激素（MSH）的分泌，而β-内啡肽可短暂逆转这种作用。内啡肽的作用又被阿片拮抗剂盐酸纳洛酮部分抑制。用这三种药物处理的细胞均表现出粗面内质网扩张和分泌颗粒含量减少，这表明有肽释放和新的合成。单独使用β-内啡肽不会使MSH分泌高于对照水平，并且在某个时间段内还会使MSH分泌减少。这些发现表明，β-内啡肽和多巴胺在神经中间叶水平上直接对MSH分泌存在复杂的相互作用。
