Voigt K H, Frank D, Düker E, Martin R, Wuttke W
Life Sci. 1983;33 Suppl 1:507-10. doi: 10.1016/0024-3205(83)90552-0.
Primary monolayer cultures of anterior or intermediate lobe (IL) cells from rat pituitaries were grown in microtiter plates, and the test substances were added after 5 days. The hormones were measured by RIAs for rat prolactin, -MSH, ACTH and beta-endorphin/beta-LPH. The dopamine (DA) inhibition of prolactin secretion was attenuated by addition of equimolar (10(-7), 10(-6)M) concentration of beta-endorphin. Furthermore, the DA effect was blocked, when the beta-endorphin-release of anterior pituitary cells was stimulated by vasopressin. In contrast, the DA induced inhibition of hormone-release of IL-cells (e.g. -MSH, ACTH and beta-endorphin) was not influenced by addition of opioids. Our results indicate, that under certain conditions (e.g. stress) the stimulated secretion of prolactin could be mediated partially by a direct action of pituitary beta-endorphin at the lactotroph. On the other hand, we found no indication for a modulation of the dopamine-induced inhibition of IL-peptides by opioids.
将大鼠垂体前叶或中间叶（IL）细胞进行原代单层培养，接种于微量滴定板中，5天后加入受试物质。采用放射免疫分析法（RIAs）测定大鼠催乳素、促黑素（-MSH）、促肾上腺皮质激素（ACTH）和β-内啡肽/β-促脂素。加入等摩尔浓度（10⁻⁷、10⁻⁶M）的β-内啡肽可减弱多巴胺（DA）对催乳素分泌的抑制作用。此外，当血管加压素刺激垂体前叶细胞释放β-内啡肽时，DA的作用被阻断。相反，添加阿片类物质并不影响DA诱导的IL细胞激素释放抑制作用（如促黑素、促肾上腺皮质激素和β-内啡肽）。我们的结果表明，在某些条件下（如应激），催乳素的刺激分泌可能部分由垂体β-内啡肽对催乳细胞的直接作用介导。另一方面，我们没有发现阿片类物质对多巴胺诱导的IL肽抑制作用有调节作用的迹象。
