多巴胺对催乳素及垂体中间叶促黑素细胞激素原衍生肽释放的抑制作用：阿片类药物的不同调节作用

Dopamine-inhibited release of prolactin and intermediate lobe-POMC-peptides: different modulation by opioids.

作者信息

Voigt K H, Frank D, Düker E, Martin R, Wuttke W

出版信息

Life Sci. 1983;33 Suppl 1:507-10. doi: 10.1016/0024-3205(83)90552-0.

PMID:6319905

Abstract

Primary monolayer cultures of anterior or intermediate lobe (IL) cells from rat pituitaries were grown in microtiter plates, and the test substances were added after 5 days. The hormones were measured by RIAs for rat prolactin, -MSH, ACTH and beta-endorphin/beta-LPH. The dopamine (DA) inhibition of prolactin secretion was attenuated by addition of equimolar (10(-7), 10(-6)M) concentration of beta-endorphin. Furthermore, the DA effect was blocked, when the beta-endorphin-release of anterior pituitary cells was stimulated by vasopressin. In contrast, the DA induced inhibition of hormone-release of IL-cells (e.g. -MSH, ACTH and beta-endorphin) was not influenced by addition of opioids. Our results indicate, that under certain conditions (e.g. stress) the stimulated secretion of prolactin could be mediated partially by a direct action of pituitary beta-endorphin at the lactotroph. On the other hand, we found no indication for a modulation of the dopamine-induced inhibition of IL-peptides by opioids.

摘要

将大鼠垂体前叶或中间叶（IL）细胞进行原代单层培养，接种于微量滴定板中，5天后加入受试物质。采用放射免疫分析法（RIAs）测定大鼠催乳素、促黑素（-MSH）、促肾上腺皮质激素（ACTH）和β-内啡肽/β-促脂素。加入等摩尔浓度（10⁻⁷、10⁻⁶M）的β-内啡肽可减弱多巴胺（DA）对催乳素分泌的抑制作用。此外，当血管加压素刺激垂体前叶细胞释放β-内啡肽时，DA的作用被阻断。相反，添加阿片类物质并不影响DA诱导的IL细胞激素释放抑制作用（如促黑素、促肾上腺皮质激素和β-内啡肽）。我们的结果表明，在某些条件下（如应激），催乳素的刺激分泌可能部分由垂体β-内啡肽对催乳细胞的直接作用介导。另一方面，我们没有发现阿片类物质对多巴胺诱导的IL肽抑制作用有调节作用的迹象。