哈维鼠肉瘤病毒p21 v-ras基因转化序列的进一步基因定位

Further genetic localization of the transforming sequences of the p21 v-ras gene of Harvey murine sarcoma virus.

作者信息

Willumsen B M, Ellis R W, Scolnick E M, Lowy D R

出版信息

J Virol. 1984 Feb;49(2):601-3. doi: 10.1128/JVI.49.2.601-603.1984.

DOI:10.1128/JVI.49.2.601-603.1984

PMID:6319763

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC255506/

Abstract

The sequences encoding the 21-kilodalton transforming protein (p21 ras) of Harvey murine sarcoma virus have previously been localized genetically to a 1.3-kilobase segment of the viral DNA (E. H. Chang, R. W. Ellis, E. M. Scolnick, and D. R. Lowy, Science 210:1249-1251, 1980). Within this segment, DNA sequence analysis has found a single open reading frame large enough to encode the viral p21 (R. Dhar, R. W. Ellis, T. Y. Shih, S. Oroszlan, B. Shapiro, J. Maizel, D. Lowy, and E. M. Scolnick, Science 217:934-937, 1982). There are three potential in-frame ATG initiation codons at the 5' end of this open reading frame. By constructing a mutant of Harvey murine sarcoma virus DNA from which the first two ATG codons of this open reading frame have been deleted, we now show by transfection of the mutant viral DNA into NIH 3T3 cells that only the third ATG codon is necessary and sufficient for synthesis of the viral p21 and for cellular transformation.

摘要

哈维鼠肉瘤病毒编码21千道尔顿转化蛋白（p21 ras）的序列先前已通过遗传学方法定位到病毒DNA的一个1.3千碱基片段上（E. H. 张、R. W. 埃利斯、E. M. 斯科尔尼克和D. R. 洛伊，《科学》210:1249 - 1251，1980年）。在这个片段内，DNA序列分析发现了一个足够大的单一开放阅读框，足以编码病毒p21（R. 达尔、R. W. 埃利斯、T. Y. 施、S. 奥罗斯兰、B. 夏皮罗、J. 迈泽尔、D. 洛伊和E. M. 斯科尔尼克，《科学》217:934 - 937，1982年）。在这个开放阅读框的5'端有三个潜在的符合读码框的ATG起始密码子。通过构建一个哈维鼠肉瘤病毒DNA突变体，其中这个开放阅读框的前两个ATG密码子已被删除，我们现在通过将突变的病毒DNA转染到NIH 3T3细胞中表明，只有第三个ATG密码子对于病毒p21的合成和细胞转化是必要且充分的。