Bentz M S, Cohen C, Demers L M, Budgeon L R
Arch Pathol Lab Med. 1982 Sep;106(9):476-80.
An immunoperoxidase technique to detect prostatic-specific acid phosphatase (PSAP) was used on specimens from 98 cases of prostatic carcinoma that were graded by both the Gleason and the Mostofi systems, to see if tumor grade correlated with amount of PSAP seen in tissue. Most tumors showed strong, diffuse cytoplasmic staining; no significant difference was seen among the various grades. Other than focal, weak staining of renal tubular epithelium, the antibody to PSAP gave uniformly negative results with a variety of normal and neoplastic tissues. In light of the great sensitivity and specificity of this technique, it potential applications include diagnosis of poorly differentiated prostatic malignant neoplasms, whether primary or metastatic.
一种检测前列腺特异性酸性磷酸酶（PSAP）的免疫过氧化物酶技术应用于98例前列腺癌标本，这些标本同时采用Gleason和Mostofi系统进行分级，以观察肿瘤分级与组织中PSAP含量是否相关。大多数肿瘤显示出强烈、弥漫性的细胞质染色；不同分级之间未见显著差异。除肾小管上皮的局灶性弱阳性染色外，PSAP抗体对各种正常和肿瘤组织均给出一致的阴性结果。鉴于该技术具有高度的敏感性和特异性，其潜在应用包括诊断低分化前列腺恶性肿瘤，无论是原发性还是转移性的。
