产气荚膜梭菌肠毒素在大鼠肝细胞中的结合定量及亚细胞分布
Quantitation of binding and subcellular distribution of Clostridium perfringens enterotoxin in rat liver cells.
作者信息
Tolleshaug H, Skjelkvåle R, Berg T
出版信息
Infect Immun. 1982 Aug;37(2):486-91. doi: 10.1128/iai.37.2.486-491.1982.
Abstract
Binding of enterotoxin from Clostridium perfringens type A was studied in suspensions of parenchymal and nonparenchymal cells from rat liver. In hepatocytes, 1.5 X 10(6) specific binding sites per cell with an association constant of 3.2 X 10(6) M-1 were found. About 1% of the added toxin was nonspecifically bound to the hepatocytes. At concentrations of toxin below 0.1 micrograms/ml, 80% of the toxin density of 7 X 10(6) cells per ml. Binding did not increase after the cells became permeable to the toxin. Subcellular fractionation in a sucrose gradient produced no evidence for binding to parts of the cell other than the plasma membrane. The degree of binding to nonparenchymal cells was less than 10% of the binding to hepatocytes.
摘要
对A型产气荚膜梭菌肠毒素与大鼠肝脏实质细胞和非实质细胞悬液的结合进行了研究。在肝细胞中，发现每个细胞有1.5×10⁶个特异性结合位点，缔合常数为3.2×10⁶M⁻¹。约1%添加的毒素非特异性地结合到肝细胞上。在毒素浓度低于0.1微克/毫升时，每毫升7×10⁶个细胞的毒素密度中有80%发生结合。细胞对毒素变得通透后，结合并未增加。在蔗糖梯度中进行亚细胞分级分离，没有证据表明毒素结合到细胞膜以外的细胞部分。与非实质细胞的结合程度不到与肝细胞结合程度的10%。
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