产气荚膜梭菌肠毒素在大鼠肝细胞中的结合定量及亚细胞分布
Quantitation of binding and subcellular distribution of Clostridium perfringens enterotoxin in rat liver cells.
作者信息
Tolleshaug H, Skjelkvåle R, Berg T
出版信息
Infect Immun. 1982 Aug;37(2):486-91. doi: 10.1128/iai.37.2.486-491.1982.
Abstract
Binding of enterotoxin from Clostridium perfringens type A was studied in suspensions of parenchymal and nonparenchymal cells from rat liver. In hepatocytes, 1.5 X 10(6) specific binding sites per cell with an association constant of 3.2 X 10(6) M-1 were found. About 1% of the added toxin was nonspecifically bound to the hepatocytes. At concentrations of toxin below 0.1 micrograms/ml, 80% of the toxin density of 7 X 10(6) cells per ml. Binding did not increase after the cells became permeable to the toxin. Subcellular fractionation in a sucrose gradient produced no evidence for binding to parts of the cell other than the plasma membrane. The degree of binding to nonparenchymal cells was less than 10% of the binding to hepatocytes.
摘要
对A型产气荚膜梭菌肠毒素与大鼠肝脏实质细胞和非实质细胞悬液的结合进行了研究。在肝细胞中，发现每个细胞有1.5×10⁶个特异性结合位点，缔合常数为3.2×10⁶M⁻¹。约1%添加的毒素非特异性地结合到肝细胞上。在毒素浓度低于0.1微克/毫升时，每毫升7×10⁶个细胞的毒素密度中有80%发生结合。细胞对毒素变得通透后，结合并未增加。在蔗糖梯度中进行亚细胞分级分离，没有证据表明毒素结合到细胞膜以外的细胞部分。与非实质细胞的结合程度不到与肝细胞结合程度的10%。
相似文献
1
Quantitation of binding and subcellular distribution of Clostridium perfringens enterotoxin in rat liver cells.产气荚膜梭菌肠毒素在大鼠肝细胞中的结合定量及亚细胞分布
Infect Immun. 1982 Aug;37(2):486-91. doi: 10.1128/iai.37.2.486-491.1982.
2
Binding of Clostridium perfringens enterotoxin to hepatocytes, small intestinal epithelial cells and Vero cells.产气荚膜梭菌肠毒素与肝细胞、小肠上皮细胞及非洲绿猴肾细胞的结合。
Acta Pathol Microbiol Immunol Scand B. 1982 Oct;90(5):377-81. doi: 10.1111/j.1699-0463.1982.tb00134.x.
3
Preparation of isolated liver endothelial cells and Kupffer cells in high yield by means of an enterotoxin.通过一种肠毒素高产制备分离的肝内皮细胞和库普弗细胞。
Exp Cell Res. 1984 Jan;150(1):194-204. doi: 10.1016/0014-4827(84)90714-6.
4
RIP1, RIP3, and MLKL Contribute to Cell Death Caused by Clostridium perfringens Enterotoxin.RIP1、RIP3 和 MLKL 导致产气荚膜梭菌肠毒素引起的细胞死亡。
mBio. 2019 Dec 17;10(6):e02985-19. doi: 10.1128/mBio.02985-19.
5
Binding of enterotoxin from Clostridium perfringens type A to liver cells in vivo and in vitro. The enterotoxin causes membrane leakage.A型产气荚膜梭菌肠毒素在体内和体外与肝细胞的结合。该肠毒素会导致膜渗漏。
Acta Pathol Microbiol Scand B. 1980 Apr;88(2):95-102. doi: 10.1111/j.1699-0463.1980.tb02612.x.
6
Binding of Clostridium perfringens [125I]enterotoxin to rabbit intestinal cells.产气荚膜梭菌[125I]肠毒素与兔肠道细胞的结合。
Biochemistry. 1980 Oct 14;19(21):4801-7. doi: 10.1021/bi00562a014.
7
Enteropathogenicity of purified Clostridium perfringens enterotoxin in the pig.猪源产气荚膜梭菌肠毒素纯化产物的肠道致病性
Am J Vet Res. 1985 Oct;46(10):2147-8.
8
Native or Proteolytically Activated NanI Sialidase Enhances the Binding and Cytotoxic Activity of Clostridium perfringens Enterotoxin and Beta Toxin.天然或经蛋白水解激活的NanI唾液酸酶增强产气荚膜梭菌肠毒素和β毒素的结合及细胞毒性活性。
Infect Immun. 2017 Dec 19;86(1). doi: 10.1128/IAI.00730-17. Print 2018 Jan.
9
Four foodborne disease outbreaks caused by a new type of enterotoxin-producing Clostridium perfringens.四起由新型产肠毒素产气荚膜梭菌引起的食源性疾病暴发。
J Clin Microbiol. 2015 Mar;53(3):859-67. doi: 10.1128/JCM.02859-14. Epub 2015 Jan 7.
10
Diversity of toxin-genotypes among Clostridium perfringens isolated from healthy and diarrheic neonatal cattle and buffalo calves.从健康和腹泻新生牛犊及水牛犊中分离出的产气荚膜梭菌毒素基因型的多样性。
Anaerobe. 2018 Feb;49:99-102. doi: 10.1016/j.anaerobe.2018.01.001. Epub 2018 Jan 4.
引用本文的文献
1
Effects of Claudin-1 on the Action of Enterotoxin in Caco-2 Cells.Claudin-1 对肠毒素在 Caco-2 细胞中作用的影响。
Toxins (Basel). 2019 Oct 9;11(10):582. doi: 10.3390/toxins11100582.
2
Animal models to study the pathogenesis of enterotoxigenic Clostridium perfringens infections.用于研究肠毒素型产气荚膜梭菌感染发病机制的动物模型。
Microbes Infect. 2012 Oct;14(12):1009-16. doi: 10.1016/j.micinf.2012.06.003. Epub 2012 Jun 17.
3
Characterization of membrane-associated Clostridium perfringens enterotoxin following pronase treatment.蛋白酶处理后膜相关产气荚膜梭菌肠毒素的特性分析
Infect Immun. 1998 Dec;66(12):5897-905. doi: 10.1128/IAI.66.12.5897-5905.1998.
4
Molecular cloning and functional characterization of the receptor for Clostridium perfringens enterotoxin.产气荚膜梭菌肠毒素受体的分子克隆与功能特性分析
J Cell Biol. 1997 Mar 24;136(6):1239-47. doi: 10.1083/jcb.136.6.1239.
5
Isolation and function of a Clostridium perfringens enterotoxin fragment.产气荚膜梭菌肠毒素片段的分离与功能
Infect Immun. 1987 Dec;55(12):2912-5. doi: 10.1128/iai.55.12.2912-2915.1987.
6
Preliminary evidence that Clostridium perfringens type A enterotoxin is present in a 160,000-Mr complex in mammalian membranes.初步证据表明，A型产气荚膜梭菌肠毒素存在于哺乳动物细胞膜中的一种160,000道尔顿的复合物中。
Infect Immun. 1989 Feb;57(2):574-81. doi: 10.1128/iai.57.2.574-581.1989.
7
Morphological alterations and changes in cellular cations induced by Clostridium perfringens type A enterotoxin in tissue culture cells.A型产气荚膜梭菌肠毒素在组织培养细胞中诱导的形态学改变及细胞阳离子变化。
Eur J Epidemiol. 1985 Dec;1(4):264-73. doi: 10.1007/BF00237101.
8
Studies of Clostridium perfringens enterotoxin action at different temperatures demonstrate a correlation between complex formation and cytotoxicity.对产气荚膜梭菌肠毒素在不同温度下作用的研究表明，复合物形成与细胞毒性之间存在关联。
Infect Immun. 1990 Sep;58(9):3109-15. doi: 10.1128/iai.58.9.3109-3115.1990.
本文引用的文献
1
Mechanism of Action of the Enteropathogenic Factor of Clostridium perfringens Type A.A 型产气荚膜梭菌肠致病因子作用机制的研究
Infect Immun. 1971 Jan;3(1):100-6. doi: 10.1128/iai.3.1.100-106.1971.
2
DETERMINATION OF EQUILIBRIUM CONSTANTS AND MAXIMUM BINDING CAPACITIES IN COMPLEX IN VITRO SYSTEMS. I. THE MAMMILLARY SYSTEM.
Bull Math Biophys. 1965 Mar;27:87-98. doi: 10.1007/BF02476471.
3
THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY.高比放射性碘-131标记人生长激素的制备
Biochem J. 1963 Oct;89(1):114-23. doi: 10.1042/bj0890114.
4
Radioiodination of enterotoxin from Clostridium perfringens type A using chloramine T.
J Appl Bacteriol. 1980 Apr;48(2):283-95. doi: 10.1111/j.1365-2672.1980.tb01228.x.
5
Ligand: a versatile computerized approach for characterization of ligand-binding systems.配体：一种用于表征配体结合系统的通用计算机化方法。
Anal Biochem. 1980 Sep 1;107(1):220-39. doi: 10.1016/0003-2697(80)90515-1.
6
Binding of enterotoxin from Clostridium perfringens type A to liver cells in vivo and in vitro. The enterotoxin causes membrane leakage.A型产气荚膜梭菌肠毒素在体内和体外与肝细胞的结合。该肠毒素会导致膜渗漏。
Acta Pathol Microbiol Scand B. 1980 Apr;88(2):95-102. doi: 10.1111/j.1699-0463.1980.tb02612.x.
7
Mechanism of action of Clostridium perfringens enterotoxin. Effects on membrane permeability and amino acid transport in primary cultures of adult rat hepatocytes.产气荚膜梭菌肠毒素的作用机制。对成年大鼠肝细胞原代培养物中膜通透性和氨基酸转运的影响。
Biochim Biophys Acta. 1980 Jan 25;595(2):264-76. doi: 10.1016/0005-2736(80)90089-9.
8
Binding of concanavalin A to isolated hepatocytes and its effect on uptake and degradation of asialo-fetuin by the cells.伴刀豆球蛋白A与分离的肝细胞的结合及其对细胞摄取和降解去唾液酸胎球蛋白的影响。
Biochem J. 1980 Sep 15;190(3):697-703. doi: 10.1042/bj1900697.
9
Kinetics of internalization and degradation of asialo-glycoproteins in isolated rat hepatocytes.去唾液酸糖蛋白在分离的大鼠肝细胞中的内化和降解动力学
Eur J Cell Biol. 1980 Dec;23(1):104-9.
10
Formation of biologically active 125 I-prolactin by enzymatic radioiodination.通过酶促放射性碘化形成生物活性125I-催乳素。
Endocrinology. 1972 Dec;91(6):1545-8. doi: 10.1210/endo-91-6-1545.
Zotero 插件