Athira Cheruplackal Karunakaran, Milton Arockiasamy Arun Prince, Reddy Avinash, Mekhemadhom Rajendrakumar Arunraj, Verma Med Ram, Kumar Ashok, Nagaleekar Viswas Konasagara, Agarwal Rajesh Kumar
Division of Veterinary Public Health, Indian Council of Agricultural Research (ICAR)- Indian Veterinary Research Institute, Izatnagar, Bareilly, 243122, U.P., India.
Division of Immunology, Virginia-Maryland College of Veterinary Medicine, University of Maryland, College Park, MD, 20740, USA.
Anaerobe. 2018 Feb;49:99-102. doi: 10.1016/j.anaerobe.2018.01.001. Epub 2018 Jan 4.
The diversity of toxin-genotypes of C. perfringens in neonatal calves was determined in this study. A total of 682 fresh faecal samples comprising 559 healthy and 123 diarrheic neonatal calves (cattle and buffalo) were collected from various farms in Northern India. The samples were processed for isolation of C. perfringens and toxin-genotyping by multiplex PCR. The overall prevalence of C. perfringens was 37.2%. The most predominant toxin-genotype was type A (59.7%) and the least prevalent was type C. There was no association between toxin genotypes and diarrhea of cattle and buffalo neonatal calves (P > .05). Also, 38 (14.6%) and 16 (6.1%) isolates out of the 259 carried enterotoxin (cpe) and beta 2 toxin (cpb2) genes, respectively. Ten different toxin-genotypes were identified, and iota toxin gene was not detected in any of the sample.
本研究确定了新生犊牛产气荚膜梭菌毒素基因型的多样性。从印度北部的各个农场收集了总共682份新鲜粪便样本，其中包括559头健康新生犊牛（牛和水牛）和123头腹泻新生犊牛。通过多重PCR对样本进行处理，以分离产气荚膜梭菌并进行毒素基因分型。产气荚膜梭菌的总体患病率为37.2%。最主要的毒素基因型是A型（59.7%），最不常见的是C型。毒素基因型与牛和水牛新生犊牛腹泻之间没有关联（P>0.05）。此外，在259株分离株中，分别有38株（14.6%）和16株（6.1%）携带肠毒素（cpe）和β2毒素（cpb2）基因。鉴定出了10种不同的毒素基因型，在任何样本中均未检测到iota毒素基因。
