Ng T B, Chung D, Li C H
Int J Pept Protein Res. 1982 Apr;19(4):343-7. doi: 10.1111/j.1399-3011.1982.tb02613.x.
A beta-endorphin-(1-27)-like peptide was isolated from bovine cerebral hemisphere extracts by gel filtration, ion-exchange chromatography, high performance liquid chromatography and paper electrophoresis. The peptide had tyrosine as the amino-terminal residue and its amino acid composition was nearly identical to that of equine pituitary beta-EP-(1-27). It had also the same mobility as equine pituitary beta-EP-(1-27) in paper electrophoresis. In radioimmunoassay and opiate receptor-binding assay, the brain peptide had 50% activity when compared with human beta-endorphin-(1-27). Evidence for the occurrence of NH2-acetylated form of beta-endorphin-(1-27) is also presented.
通过凝胶过滤、离子交换色谱法、高效液相色谱法和纸电泳从牛脑半球提取物中分离出一种β-内啡肽-(1 - 27)样肽。该肽以酪氨酸作为氨基末端残基，其氨基酸组成与马垂体β-EP-(1 - 27)几乎相同。在纸电泳中，它与马垂体β-EP-(1 - 27)也具有相同的迁移率。在放射免疫测定和阿片受体结合测定中，与人类β-内啡肽-(1 - 27)相比，脑肽具有50%的活性。还提供了β-内啡肽-(1 - 27)的NH2-乙酰化形式存在的证据。
