Bauer G, Höfler P, Simon M
J Biol Chem. 1982 Oct 10;257(19):11405-10.
Serum contains a factor that induces Epstein-Barr virus antigens in latently infected human lymphoblastoid cell lines and that cooperates with chemical inducers. Here we report the purification of a protein that is responsible for the effect. Using ammonium sulphate precipitation, chromatography on DEAE-agarose and cellulose, molecular sieve chromatography on Bio-Gel A-5, and glycerol gradient centrifugation, the factor was enriched about 300,000-fold compared to calf serum. Under neutral conditions, the protein chromatographed as a high molecular weight protein of about 5.5 X 10(5) both in its active and inactive form. Both forms of the molecule sedimented at about 7 s. The factor is an acidic protein with a pI of 5. It seems to be composed of high molecular and low molecular weight subunits. Nanogram amounts of purified factor are sufficient for measurable inducing effects.
血清中含有一种因子，它能在潜伏感染的人淋巴母细胞系中诱导EB病毒抗原，并能与化学诱导剂协同作用。在此，我们报告了一种负责该效应的蛋白质的纯化过程。通过硫酸铵沉淀、DEAE -琼脂糖和纤维素柱层析、Bio - Gel A - 5分子筛层析以及甘油梯度离心，与小牛血清相比，该因子被富集了约300,000倍。在中性条件下，该蛋白质以约5.5×10⁵的高分子量蛋白质形式进行层析，其活性和非活性形式均如此。该分子的两种形式在约7 s处沉降。该因子是一种pI为5的酸性蛋白质。它似乎由高分子量和低分子量亚基组成。纳克量的纯化因子就足以产生可测量的诱导效应。
