Bauer G, Höfler P, Simon M
J Biol Chem. 1982 Oct 10;257(19):11411-5.
Purified Epstein-Barr virus-inducing factor shows all the properties previously described for crude serum, i.e. inducing activity, cooperative activity with the inducers 12-O-tetradecanoyl-phorbol-13-acetate, 5'-iodo-2'-deoxyuridine, N-butyric acid, anti-IgM, and the need to be activated by pH shock. A small subpopulation of untreated factor molecules is fully active, however. The factor is a protein that is characterized by considerable heat resistance and sensitivity to reducing agents. It does not seem to require sugar residues or lipid for its activity. Activation occurs both at alkaline and acidic pH. Inactive and activated factor differ in their sensitivity to trypsin and in the degree of sensitivity to reducing agents. A change of conformation within the molecule seems to be the biochemical basis for the activation reaction.
纯化的爱泼斯坦 - 巴尔病毒诱导因子具有先前描述的粗血清的所有特性，即诱导活性、与诱导剂12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯、5'-碘 - 2'-脱氧尿苷、正丁酸、抗IgM的协同活性，以及需要通过pH休克激活。然而，一小部分未处理的因子分子是完全有活性的。该因子是一种蛋白质，其特征是具有相当高的耐热性和对还原剂的敏感性。其活性似乎不需要糖残基或脂质。在碱性和酸性pH下均可发生激活。无活性和有活性的因子在对胰蛋白酶的敏感性以及对还原剂的敏感程度上有所不同。分子内构象的变化似乎是激活反应的生化基础。
