人类α-L-岩藻糖苷酶结构基因在1号染色体上的定位以及在2号染色体上的同源位点。

Chromosome 1 localization of the human alpha-L-fucosidase structural gene with a homologous site on chromosome 2.

作者信息

Fowler M L, Nakai H, Byers M G, Fukushima H, Eddy R L, Henry W M, Haley L L, O'Brien J S, Shows T B

出版信息

Cytogenet Cell Genet. 1986;43(1-2):103-8. doi: 10.1159/000132304.

Abstract

Two cDNA clones coding for human alpha-L-fucosidase, one from the coding region and the other primarily from the 3' untranslated region, were used to map the location of the alpha-L-fucosidase gene. Southern filter analysis of somatic cell hybrid lines mapped the structural gene to the short arm of human chromosome 1, and in situ hybridization to chromosomes of human leukocytes further localized the homologous area to the 1p36.1----p34.1 region, with the most likely location being the distal region of 1p34. Further Southern filter analysis detected a second site of homology on chromosome 2. This alpha-L-fucosidase-like site has been designated FUCA1L.

摘要

两个编码人α-L-岩藻糖苷酶的cDNA克隆，一个来自编码区，另一个主要来自3'非翻译区，被用于绘制α-L-岩藻糖苷酶基因的定位图。对体细胞杂种系进行的Southern印迹分析将结构基因定位到人类染色体1的短臂上，而对人类白细胞染色体进行的原位杂交进一步将同源区域定位到1p36.1----p34.1区域，最可能的位置是1p34的远端区域。进一步的Southern印迹分析在染色体2上检测到第二个同源位点。这个α-L-岩藻糖苷酶样位点被命名为FUCA1L。