Simon W H, Wohl D L
Connect Tissue Res. 1982;9(4):227-32. doi: 10.3109/03008208209160266.
A method is described for accurate and reproducible determination of wet and dry weights of small articular cartilage samples. The water content of the articular cartilage samples before and after treatment with selective degradative enzymes and "artificial fibrillation" was determined. It was found that the water content of articular cartilage can be altered by changing the physical and chemical composition of articular cartilage. The combination of protein polysaccharide degradation and "fibrillation" causes an increase in articular cartilage water content, while collagenase and the combination of collagenase and "fibrillation" causes a decrease. The relationship between these findings and function of normal and osteoarthritic cartilage is discussed.
本文描述了一种准确且可重复测定小关节软骨样本湿重和干重的方法。测定了经选择性降解酶处理和“人工纤维化”前后关节软骨样本的含水量。结果发现，通过改变关节软骨的物理和化学组成可以改变其含水量。蛋白多糖降解与“纤维化”的联合作用导致关节软骨含水量增加，而胶原酶以及胶原酶与“纤维化”的联合作用则导致含水量降低。讨论了这些发现与正常和骨关节炎软骨功能之间的关系。
