Kalcheim C, Duksin D, Vogel Z
Neurosci Lett. 1982 Aug 31;31(3):265-70. doi: 10.1016/0304-3940(82)90031-3.
Coculturing of rat embryonic muscle cells with spinal cord explants resulted in the formation of large numbers of acetylcholine receptor aggregates on the myotube surface, compared to those found on muscle cells grown in the absence of nervous tissue. Remarkably fewer receptor aggregates were formed when, upon addition of nerve explants, these cocultures were treated with either cis-hydroxyproline (a specific inhibitor of collagen production) or collagenase. The possibility is raised that collagen participates in the aggregation of acetylcholine receptors and in synapse formation.
与在无神经组织条件下生长的肌肉细胞相比，将大鼠胚胎肌肉细胞与脊髓外植体共培养，会导致肌管表面形成大量乙酰胆碱受体聚集体。值得注意的是，当加入神经外植体后，用顺式羟脯氨酸（一种胶原蛋白产生的特异性抑制剂）或胶原酶处理这些共培养物时，形成的受体聚集体明显减少。这就提出了一种可能性，即胶原蛋白参与乙酰胆碱受体的聚集和突触形成。
