Jay J C, Barald K F
J Neurosci Methods. 1985 Nov-Dec;15(3):229-34. doi: 10.1016/0165-0270(85)90102-5.
Suspension culture of single adult rat flexor digitorum brevis (FDB) muscle fibres in Vitrogen, a purified collagen, on tissue culture plastic or glass with mesh ring supports is superior to culture upon other substrates including collagen-, laminin-, or Vitrogen-coated tissue culture plastic. The Vitrogen gel-fibre mixture which attaches to glass or plastic provides at least 10 times more fibres per dish than does plating fibres on other substrates. Use of Vitrogen gel permits variable plating densities and the production of adequate numbers of cultures for long-term experimental comparisons of acetylcholinesterase (AChE) and rhodamine-alpha-bungarotoxin (RBTX) distribution on muscle fibres. Use of 40 micrograms/ml ovotransferrin (OT) instead of chick embryo extract in the culture medium significantly improves long-term survival. Cultured fibres, with or without the addition of ventral spinal cord explants. may also be examined with electrophysiological techniques.
在组织培养塑料或玻璃上，利用网状环支撑物，将成年大鼠单一趾短屈肌（FDB）肌纤维悬浮培养于纯化胶原蛋白Vitrogen中，这种方法优于在包括胶原蛋白、层粘连蛋白或Vitrogen包被的组织培养塑料等其他基质上进行培养。附着于玻璃或塑料上的Vitrogen凝胶-纤维混合物，每培养皿提供的纤维数量比在其他基质上接种纤维至少多10倍。使用Vitrogen凝胶可实现可变的接种密度，并能产生足够数量的培养物，用于对肌纤维上乙酰胆碱酯酶（AChE）和罗丹明-α-银环蛇毒素（RBTX）分布进行长期实验比较。在培养基中使用40微克/毫升的卵转铁蛋白（OT）代替鸡胚提取物，可显著提高长期存活率。无论是否添加腹侧脊髓外植体，培养的纤维都可用电生理技术进行检测。
