非组蛋白染色质蛋白HMG-1对纯化DNA酶促消化的影响。
Influence of nonhistone chromatin protein HMG-1 on the enzymatic digestion of purified DNA.
作者信息
Shastri K, Isackson P J, Fishback J L, Land M D, Reeck G R
出版信息
Nucleic Acids Res. 1982 Aug 25;10(16):5059-72. doi: 10.1093/nar/10.16.5059.
Abstract
The effect of chicken erythrocyte High Mobility Group protein 1 (HMG-1) on the enzymatic hydrolysis of purified double-stranded and single-stranded bacteriophage lambda DNA was studied. HMG-1 was found to inhibit the digestion of single- and double-stranded DNA by S1 nuclease and DNase I, respectively. HMG-I increased the rate of hydrolysis of double-stranded DNA by micrococcal nuclease, particularly at low HMG-1/DNA ratios, and had little effect on the hydrolysis of single-stranded DNA by micrococcal nucleases, even at high HMG-1 DNA ratios. We also present a semi-quantitative estimate that HMG-1 and HMG-2 occur in chromatin from rapidly dividing, cultured rat hepatoma cells at about 8 times the level that they occur in adult rat liver chromatin.
摘要
研究了鸡红细胞高迁移率族蛋白1（HMG-1）对纯化的双链和单链噬菌体λDNA酶促水解的影响。发现HMG-1分别抑制S1核酸酶和DNase I对单链和双链DNA的消化。HMG-I提高了微球菌核酸酶对双链DNA的水解速率，特别是在低HMG-1/DNA比率时，而即使在高HMG-1 DNA比率下，对微球菌核酸酶对单链DNA的水解也几乎没有影响。我们还给出了一个半定量估计，即快速分裂的培养大鼠肝癌细胞染色质中的HMG-1和HMG-2含量约为成年大鼠肝脏染色质中含量的8倍。
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